| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mariotti, Isabel G (17)
|
NIGVST |
25.50 |
26.78 |
1.28 |
| 2 |
Snayczuk, Madison J (14)
|
NIAAAC |
26.64 |
27.51 |
0.87 |
| 3 |
Staba, Lauren M (14)
|
NIGVST |
26.83 |
28.24 |
1.41 |
| 4 |
Ditchey, Lauren E (16)
|
NISWAT |
28.16 |
28.44 |
0.28 |
| 5 |
Hurd, Taylor M (15)
|
NIGVST |
27.98 |
29.42 |
1.44 |
| 6 |
Crawford, Loralai M (15)
|
NIGVST |
28.94 |
29.44 |
0.50 |
| 7 |
Delaney, Carley A (14)
|
NISWAT |
28.97 |
29.90 |
0.93 |
| 8 |
Harrington, Porsha M (13)
|
NIAAAC |
30.03 |
30.13 |
0.10 |
| 9 |
Stang, Megan O (17)
|
NIGWST |
29.24 |
30.22 |
0.98 |
| 10 |
Delpriore, Riley A (17)
|
NIGWST |
29.54 |
30.31 |
0.77 |
| 11 |
Miller, Leiya M (16)
|
NIAAAC |
28.92 |
30.60 |
1.68 |
| 12 |
Slavinski, Anna C (13)
|
NIFKVL |
30.12 |
31.25 |
1.13 |
| 13 |
Stang, Hayley M (14)
|
NIGWST |
33.24 |
33.16 |
-0.08 |
| 14 |
Dinse, Lilia P (15)
|
NISWAT |
33.23 |
33.24 |
0.01 |
| 15 |
Castellano, Madison A (14)
|
NIGVST |
36.02 |
35.14 |
-0.88 |
| 16 |
Merrill, Cameron R (13)
|
NIGWST |
34.66 |
36.18 |
1.52 |
| 17 |
Romance, Payton E (13)
|
NIAAAC |
36.85 |
36.85 |
0.00 |
| 18 |
Weiss, Emmie S (13)
|
NISWAT |
48.55 |
45.19 |
-3.36 |
| 19 |
Greaves, Olivia R (13)
|
NISWAT |
46.36 |
46.61 |
0.25 |
|
Yarbour, Jem O (14)
|
NISAAC |
30.00 |
NS |
- |