| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Boberg, Jonathan C (16)
|
NISWAT |
24.59 |
24.92 |
0.33 |
| 2 |
Cochran, Silas B (16)
|
NIGVST |
25.96 |
25.78 |
-0.18 |
| 3 |
Hand, Gavin J (15)
|
NIGVST |
25.66 |
26.23 |
0.57 |
| 4 |
Farrington, Aeden B (15)
|
NIFKVL |
26.79 |
27.04 |
0.25 |
| 5 |
Yetter, Austin A (13)
|
NISWAT |
26.85 |
27.19 |
0.34 |
| 6 |
Zywiczynski, Nathan J (14)
|
NIAAAC |
31.56 |
28.02 |
-3.54 |
| 7 |
Merrill, Caleb J (16)
|
NIGWST |
28.00 |
28.65 |
0.65 |
| 8 |
Safford, Christian T (13)
|
NISWAT |
33.05 |
31.84 |
-1.21 |
| 9 |
Harrington, John C (13)
|
NIGVST |
34.43 |
33.05 |
-1.38 |
| 10 |
Jordan, Jack A (13)
|
NIGVST |
32.16 |
33.14 |
0.98 |
| 11 |
Krajewski, Benjamin R (13)
|
NIGWST |
34.33 |
34.11 |
-0.22 |
| 12 |
Coble, Tyler R (13)
|
NIGVST |
34.84 |
34.15 |
-0.69 |
| 13 |
Simmons, Justin D (13)
|
NISWAT |
41.40 |
41.36 |
-0.04 |