| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Golden, Elizabeth (10)
|
ZZESYS |
35.25 |
33.85 |
-1.40 |
| 2 |
Enderton, Lauren (10)
|
LOCK |
39.14 |
39.13 |
-0.01 |
| 3 |
Borrero, Chloe (10)
|
ZZESYS |
38.19 |
39.98 |
1.79 |
| 4 |
Moats, Rachel (10)
|
COR |
40.45 |
40.02 |
-0.43 |
| 5 |
Schuler, Allison (10)
|
KTYC |
42.67 |
42.04 |
-0.63 |
| 6 |
Weber, Robin (10)
|
MAPY |
38.68 |
42.19 |
3.51 |
| 7 |
Bulger, Olivia (10)
|
ZZESYS |
44.47 |
44.18 |
-0.29 |
| 8 |
Amon, Madison (10)
|
ZZESYS |
41.68 |
45.13 |
3.45 |
| 9 |
Olivieri, Stella C (9)
|
NICAQY |
46.29 |
45.26 |
-1.03 |
| 10 |
Gracey-Dalton, Karsyn A (10)
|
NIBRAD |
47.84 |
45.38 |
-2.46 |
| 11 |
Lee, Kierstin O (10)
|
GBY |
45.13 |
47.34 |
2.21 |
| 12 |
Hren, Stella (9)
|
YJET |
45.09 |
47.40 |
2.31 |
| 13 |
Waite, Leah (10)
|
YJET |
47.34 |
47.60 |
0.26 |
| 14 |
Richardson, Emma (10)
|
IHYS |
50.75 |
48.17 |
-2.58 |
| 15 |
Elling, Elizabeth (9)
|
CSAY |
50.38 |
49.53 |
-0.85 |
| 16 |
Fraas, Lydia (9)
|
IHYS |
48.79 |
50.40 |
1.61 |
| 17 |
Clark, Alij'nayah (10)
|
YJET |
46.68 |
50.59 |
3.91 |
| 18 |
Kauffman, Brooke (9)
|
LOCK |
50.67 |
51.10 |
0.43 |
| 19 |
Wang, Grace (10)
|
IHYS |
47.22 |
51.46 |
4.24 |
| 20 |
Shimmel, Zoey (9)
|
YJET |
50.79 |
51.78 |
0.99 |
| 21 |
Roddy, Gabrielle (9)
|
BAYT |
49.45 |
51.84 |
2.39 |
| 22 |
Balsdon, Audrey (9)
|
KTYC |
56.40 |
52.33 |
-4.07 |
| 23 |
Whitley-Grassi, Ava (10)
|
IHYS |
49.38 |
53.13 |
3.75 |
| 24 |
Sanders, Mia (10)
|
ZZESYS |
50.70 |
54.58 |
3.88 |
| 25 |
Zorich, Georgia (9)
|
KTYC |
|
54.59 |
- |
| 26 |
Kappel, Alexandra (10)
|
IHYS |
54.74 |
55.17 |
0.43 |
| 27 |
Benedict, Claire (9)
|
KTYC |
58.14 |
58.22 |
0.08 |
| 28 |
Ezenwa, Uche (9)
|
IHYS |
1:01.92 |
59.07 |
-2.85 |
| 29 |
Galley, Maggie (9)
|
LOCK |
1:04.95 |
1:00.79 |
-4.16 |
| 30 |
Hoffman, Charlotte (9)
|
IHYS |
1:13.13 |
1:09.29 |
-3.84 |
|
Brady, Beatrice (9)
|
KTYC |
|
DQ |
- |
|
Maloney, Ava (9)
|
ZZESYS |
52.19 |
DQ |
- |