| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brink, Keelyn R (11)
|
MRWISC |
37.47 |
37.04 |
-0.43 |
| 2 |
Pupke, Cameron R (11)
|
MRWISC |
40.08 |
38.39 |
-1.69 |
| 3 |
Benavides, Genesis S (12)
|
MRWISC |
42.98 |
39.57 |
-3.41 |
| 4 |
Neary, Madison G (12)
|
MRWISC |
50.63 |
42.72 |
-7.91 |
| 5 |
Harrison, Violet T (12)
|
MRWISC |
43.32 |
43.77 |
0.45 |
| 6 |
Hayes, Cassidy C (11)
|
MRWISC |
47.52 |
43.79 |
-3.73 |
| 7 |
Kunca, Mikalah A (11)
|
MRCFJV |
43.62 |
44.80 |
1.18 |
| 8 |
Amato, Esther (11)
|
MRCFJV |
50.87 |
47.21 |
-3.66 |
| 9 |
Yates, Jordyn A (12)
|
MRMHHW |
50.62 |
47.61 |
-3.01 |
| 10 |
Madison, Lucinda M (11)
|
MRWISC |
51.99 |
52.29 |
0.30 |
| 11 |
Ali, Katina L (11)
|
MRWISC |
53.22 |
52.37 |
-0.85 |
| 12 |
Klempner, Morgan (12)
|
MRCFJV |
58.18 |
55.65 |
-2.53 |
| 13 |
Cabrera-Hill, Angelese A (12)
|
MRRFAC |
1:00.32 |
59.93 |
-0.39 |