| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gula, Mackenzie M (13)
|
MRPBAC |
25.89 |
26.04 |
0.15 |
| 2 |
Vos, Brooke C (13)
|
MRRFAC |
27.73 |
27.11 |
-0.62 |
| 3 |
O'Brien, Kate (14)
|
MRWISC |
26.11 |
27.19 |
1.08 |
| 4 |
Hannon, Morgan A (13)
|
MRHVD |
27.84 |
27.63 |
-0.21 |
| 5 |
Lloyd, Annina (14)
|
MRHVD |
28.81 |
28.54 |
-0.27 |
| 6 |
Hedman, Else (14)
|
MRCFJV |
28.97 |
28.60 |
-0.37 |
| 7 |
Hayes, Kayla (13)
|
MRWISC |
28.81 |
28.97 |
0.16 |
| 8 |
DeFranco, Ava (14)
|
MRWISC |
28.92 |
29.22 |
0.30 |
| 9 |
Dabo, Mariama F (13)
|
MRHVD |
27.99 |
29.46 |
1.47 |
| 10 |
Chicaiza, Natali (14)
|
MRCFJV |
28.98 |
29.51 |
0.53 |
| 11 |
Sweeney, Madelyn J (14)
|
MRHVD |
28.77 |
29.59 |
0.82 |
| 12 |
Jimenez, Hermione E (13)
|
MRRFAC |
29.07 |
29.74 |
0.67 |
| 13 |
McKnight, Natalie (14)
|
NIAMTS |
29.85 |
30.05 |
0.20 |
| 14 |
Civitano, Emily A (13)
|
MRHVD |
30.54 |
30.66 |
0.12 |
| 15 |
Hewitt, Allison C (13)
|
MRNDAC |
31.15 |
31.30 |
0.15 |
| 16 |
Klose, Shayna (14)
|
NIAMTS |
30.35 |
31.48 |
1.13 |
| 17 |
Perez Quiroz, Brianna Y (14)
|
MRMHHW |
31.63 |
31.94 |
0.31 |
| 18 |
McCluskey, Jayden K (13)
|
MRNDAC |
31.66 |
32.14 |
0.48 |
| 19 |
Cabrera Zavala, Alexa M (13)
|
MRMHHW |
31.69 |
32.19 |
0.50 |
| 20 |
Edelmann, Bryn (13)
|
MRCFJV |
|
32.46 |
- |
| 21 |
McGuinness, Megan I (13)
|
MRWISC |
32.82 |
32.48 |
-0.34 |
| 22 |
Barbecho, Nathalie (14)
|
MRRFAC |
32.69 |
33.16 |
0.47 |
| 23 |
Iza, Zamira (14)
|
MRCFJV |
31.28 |
33.61 |
2.33 |
| 24 |
Sledzik, Emma (14)
|
MRCFJV |
34.72 |
34.18 |
-0.54 |
| 25 |
Cabrera Zavala, Ashley D (14)
|
MRMHHW |
34.89 |
34.41 |
-0.48 |
| 26 |
Santos-Perez, Debora A (14)
|
MRMHHW |
34.10 |
34.46 |
0.36 |
| 27 |
Roberts, Cassandra (13)
|
MRWISC |
34.94 |
34.98 |
0.04 |
| 28 |
Kasnia-Herman, Kaylani G (13)
|
MRRFAC |
33.09 |
35.84 |
2.75 |
|
Meek, Stella G (13)
|
MRNDAC |
38.53 |
NS |
- |