| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Watson, William C (17)
|
NCSTAR |
49.02 |
52.31 |
3.29 |
| 2 |
Hering, Tanner A (18)
|
NCSGSA |
50.57 |
52.56 |
1.99 |
| 3 |
Hering, Flinn M (16)
|
NCSGSA |
52.39 |
55.18 |
2.79 |
| 4 |
Cantrell, Eric J (17)
|
NCSGSA |
53.96 |
55.64 |
1.68 |
| 5 |
Smelzer, Peter B (16)
|
NCSGSA |
55.59 |
57.03 |
1.44 |
| 6 |
Mikuta, Christopher R (18)
|
NCSGSA |
53.02 |
57.13 |
4.11 |
| 7 |
Reilly, John W (15)
|
NCSFSC |
57.10 |
57.26 |
0.16 |
| 8 |
Keaney, Reilly E (16)
|
NCSGSA |
53.05 |
57.90 |
4.85 |
| 9 |
Cooper, William R (17)
|
NCSSS |
53.99 |
59.22 |
5.23 |
| 10 |
Covington, Charles B (13)
|
NCUN-0 |
58.50 |
1:00.09 |
1.59 |
| 11 |
Weinshel, Samuel N (16)
|
NCSSS |
58.72 |
1:00.87 |
2.15 |
| 12 |
Ramos, Noah R (15)
|
NCSFSC |
58.84 |
1:01.21 |
2.37 |
| 13 |
Dempsey, Nolan Z (17)
|
NCSSS |
55.74 |
1:01.41 |
5.67 |
| 14 |
Ramos, John M (15)
|
NCSFSC |
1:00.34 |
1:01.75 |
1.41 |
| 15 |
Holian, Tanner J (14)
|
NCSGSA |
1:10.28 |
1:03.55 |
-6.73 |
| 16 |
Schilling, Christopher A (13)
|
NCSGSA |
1:04.80 |
1:04.16 |
-0.64 |
| 17 |
Smelzer, Luke R (15)
|
NCSGSA |
1:05.28 |
1:04.53 |
-0.75 |
| 18 |
Jones, Griffin D (13)
|
NCSFSC |
1:06.60 |
1:04.66 |
-1.94 |
| 19 |
Glebus, Christopher P (15)
|
NCSGSA |
1:13.88 |
1:06.47 |
-7.41 |
| 20 |
Teoh, Reid P (14)
|
NCSTAR |
1:10.99 |
1:09.47 |
-1.52 |
| 21 |
Warrick, James C (13)
|
NCSGSA |
1:15.56 |
1:13.30 |
-2.26 |
| 22 |
Clark, Quintin S (13)
|
NCSTAR |
1:12.63 |
1:13.73 |
1.10 |
| 23 |
Epps, Dawson A (14)
|
VADYST |
1:30.72 |
1:19.24 |
-11.48 |
| 24 |
Leach, Benjamin J (9)
|
VADYST |
1:39.48 |
1:32.19 |
-7.29 |