| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smith, Holden R (16)
|
KYRPC |
46.37 |
46.22 |
-0.15 |
| 2 |
Gifford, Alexander J (17)
|
NJWFY |
46.73 |
46.55 |
-0.18 |
| 3 |
Shaheen, Jonathan M (18)
|
GACCAC |
46.42 |
46.78 |
0.36 |
| 4 |
Shourds, Luke B (17)
|
KYRACE |
47.60 |
46.92 |
-0.68 |
| 5 |
Miller, Reid H (16)
|
NCMAC |
48.00 |
47.11 |
-0.89 |
| 6 |
Lane, Nicky J (18)
|
NJLHY |
46.86 |
47.24 |
0.38 |
| 7 |
Williams, Taylor R (17)
|
SEMTYS |
47.30 |
47.63 |
0.33 |
| 8 |
Franklin, Alexander J (16)
|
VASEVA |
48.29 |
47.64 |
-0.65 |
| 9 |
Soares, Nicholas C (17)
|
MAEMAC |
48.46 |
47.71 |
-0.75 |
| 10 |
Henkel, Bryce R (17)
|
ADCPP |
47.85 |
48.00 |
0.15 |
| 11 |
Neff, Ethan L (17)
|
AMPWRS |
48.40 |
48.36 |
-0.04 |