| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bugel, Talia M (14)
|
AMRXA |
2:19.75 |
2:19.47 |
-0.28 |
| 1 |
Milliron, Marissa J (14)
|
AMANSC |
2:52.23 |
2:38.51 |
-13.72 |
| 2 |
Shaffer, Elizabeth L (13)
|
AMNHA |
2:21.51 |
2:27.51 |
6.00 |
| 2 |
Bekelja-Wagner, Angie M (13)
|
AMNHA |
2:49.71 |
2:50.34 |
0.63 |
| 3 |
Allen, Delaney R (14)
|
AMRXA |
2:43.91 |
2:33.05 |
-10.86 |
| 3 |
Eaton, Samantha G (14)
|
AMHD |
3:07.23 |
2:52.16 |
-15.07 |
| 4 |
Cribbs, Mila E (13)
|
AMPRA |
2:06.91 |
2:37.46 |
30.55 |
| 4 |
Paul, Lillian L (13)
|
AMHD |
2:53.25 |
3:02.95 |
9.70 |
| 5 |
Medjimorec, Megan E (14)
|
AMPRA |
2:12.32 |
2:37.48 |
25.16 |
| 5 |
Boronski, Alana C (13)
|
AMANSC |
3:09.79 |
3:06.64 |
-3.15 |
| 6 |
O'Connor, Brooklyn E (13)
|
AMRXA |
2:34.57 |
2:37.84 |
3.27 |
| 6 |
Watrobsky, Katelyn E (13)
|
AMHD |
3:17.98 |
3:07.46 |
-10.52 |
| 7 |
Dube, Madeline K (13)
|
AMNHA |
2:43.75 |
2:40.03 |
-3.72 |
| 7 |
Burton, Salah M (14)
|
AMHD |
3:15.98 |
3:29.78 |
13.80 |
| 8 |
An, Emma K (13)
|
AMHD |
2:43.10 |
2:40.13 |
-2.97 |
| 9 |
Rowe, Emma E (14)
|
AMPRA |
2:27.44 |
2:48.97 |
21.53 |
|
Michael, Lauren C (13)
|
AMHD |
3:16.98 |
DQ |
- |
|
Lange, Paige M (13)
|
AMNHA |
3:14.21 |
NS |
- |
|
Gurchak, Katrina J (14)
|
AMNHA |
2:40.67 |
NS |
- |