| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Howell, Owen Y (12)
|
AMKW |
39.52 |
38.63 |
-0.89 |
| 2 |
Stefanowicz, Milo A (11)
|
AMSVSC |
39.94 |
38.89 |
-1.05 |
| 3 |
Kimmel, Nathaniel D (12)
|
AMUN-R |
44.74 |
41.60 |
-3.14 |
| 4 |
Tucker, Justin K (12)
|
AMCSC |
45.16 |
42.97 |
-2.19 |
| 5 |
Powell, Trenten J (12)
|
AMBPR |
43.80 |
44.38 |
0.58 |
| 6 |
Rondon, Andre S (12)
|
AMSFAC |
52.74 |
47.74 |
-5.00 |
| 7 |
Wilson, Mac O (11)
|
AMSVSC |
50.68 |
48.51 |
-2.17 |
| 8 |
Kolbe, Matthew J (12)
|
AMUN-R |
50.36 |
50.23 |
-0.13 |
| 9 |
Alborzi, Arman N (11)
|
AMKW |
55.02 |
53.01 |
-2.01 |
| 10 |
Bedell, Alexander J (12)
|
AMSVSC |
1:20.11 |
53.27 |
-26.84 |
| 11 |
Tarbert, Christopher J (12)
|
AMBPR |
57.72 |
54.94 |
-2.78 |
| 12 |
Gibson, Kaden C (11)
|
AMMMSA |
1:04.99 |
55.15 |
-9.84 |
| 13 |
Royhab, Ronald B (12)
|
AMSVSC |
53.39 |
55.42 |
2.03 |
| 14 |
Seeley, Dylan M (12)
|
AMSVSC |
1:02.21 |
56.68 |
-5.53 |
| 15 |
Anderson, Kade R (11)
|
AMGCY |
1:18.81 |
1:07.64 |
-11.17 |
| 16 |
Rodriguez, Alejandro D (11)
|
AMPTSC |
58.19 |
1:17.23 |
19.04 |
|
Lee, Isaac N (11)
|
AMPTSC |
1:16.19 |
DQ |
- |
|
Keller, Regis W (11)
|
AMGPAC |
1:10.77 |
DQ |
- |
|
Phillips, Owen B (12)
|
AMWHAT |
1:36.55 |
DQ |
- |