| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Berg, Madeline M (12)
|
AMSFAC |
36.15 |
33.35 |
-2.80 |
| 2 |
Schrecongost, Kira M (12)
|
AMKW |
33.49 |
33.81 |
0.32 |
| 3 |
Alessio, Madison A (12)
|
AMEFSC |
40.79 |
37.54 |
-3.25 |
| 4 |
Massella, Mollie J (11)
|
AMSRST |
34.23 |
37.93 |
3.70 |
| 5 |
Ciez, Addie E (11)
|
AMUNTY |
39.43 |
38.43 |
-1.00 |
| 6 |
Moffat, Madison O (12)
|
AMBPR |
41.65 |
38.46 |
-3.19 |
| 7 |
Haas, Abigail D (11)
|
AMKW |
40.02 |
38.78 |
-1.24 |
| 8 |
Stillwagon, Ella J (11)
|
AMGCY |
37.21 |
38.81 |
1.60 |
| 9 |
Price, Brooke A (11)
|
AMSPY |
42.15 |
40.53 |
-1.62 |
| 10 |
Grant, Season O (11)
|
AMSRST |
37.38 |
41.04 |
3.66 |
| 11 |
Van Norman, Ella C (11)
|
AMGLAC |
46.94 |
41.31 |
-5.63 |
| 12 |
Augustynowicz, Macie A (11)
|
AMPTSC |
45.09 |
41.55 |
-3.54 |
| 13 |
Guilinger, Marina L (11)
|
AMBWA |
1:21.92 |
42.41 |
-39.51 |
| 14 |
DeVito, Mia (12)
|
AMCSC |
52.70 |
44.42 |
-8.28 |
| 15 |
Gurganus, Addison M (12)
|
AMBWA |
49.15 |
44.50 |
-4.65 |
| 16 |
Marculescu, Cristina (12)
|
AMKW |
53.98 |
44.83 |
-9.15 |
| 17 |
Jackson, Julia R (11)
|
AMFAST |
46.03 |
46.62 |
0.59 |
| 18 |
Roberts, Grace L (11)
|
AMWAAC |
50.97 |
47.70 |
-3.27 |
| 19 |
Sins, Hannah R (12)
|
AMBWA |
54.16 |
47.92 |
-6.24 |
| 20 |
Schwendemann, Lola P (11)
|
AMSVSC |
54.40 |
50.75 |
-3.65 |
| 21 |
Bett, Carly M (12)
|
AMGCY |
51.77 |
50.93 |
-0.84 |
| 22 |
Gruendler, Katherine G (11)
|
AMBWA |
50.25 |
51.16 |
0.91 |
| 23 |
Vitale, Sarah B (11)
|
AMSASC |
1:12.67 |
54.02 |
-18.65 |
| 24 |
Shelly, Zia R (11)
|
AMWHAT |
56.48 |
55.05 |
-1.43 |
| 25 |
DeJesus, Sienna J (11)
|
AMUFAS |
56.99 |
55.53 |
-1.46 |
| 26 |
Conway, Kaylie M (11)
|
AMBPR |
59.11 |
58.68 |
-0.43 |
| 27 |
Vaughan, Jordan C (11)
|
AMSVSC |
1:05.49 |
1:01.13 |
-4.36 |
| 28 |
Vitale, Jenna M (11)
|
AMSASC |
1:01.25 |
1:02.34 |
1.09 |
|
Frisbee, Alexis C (11)
|
AMSASC |
1:25.66 |
DQ |
- |