| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stetor, Wyatt R (10)
|
AMUPEA |
34.99 |
34.68 |
-0.31 |
| 2 |
Howard, Thomas A (10)
|
AMMLAC |
35.72 |
36.08 |
0.36 |
| 3 |
Semenov, Ethan B (10)
|
AMJCCS |
37.74 |
36.95 |
-0.79 |
| 4 |
Turner, Joshua O (10)
|
AMRACE |
40.60 |
39.71 |
-0.89 |
| 5 |
O'Keefe, Logan E (9)
|
AMANSC |
41.66 |
39.86 |
-1.80 |
| 6 |
Liang, Nathan (10)
|
AMNHA |
40.16 |
39.96 |
-0.20 |
| 7 |
Pickett, Noah M (10)
|
AMMAC |
42.30 |
41.03 |
-1.27 |
| 8 |
Lozowski, Nicholas R (10)
|
AMSVSC |
40.59 |
41.29 |
0.70 |
| 9 |
Agens, Nathan A (10)
|
AMKW |
41.23 |
42.46 |
1.23 |
| 10 |
Watkins, Reece A (10)
|
AMANSC |
48.02 |
42.71 |
-5.31 |
| 11 |
Crane, Ryan T (10)
|
AMANSC |
45.31 |
43.13 |
-2.18 |
| 12 |
Pickett, Christopher B (8)
|
AMMAC |
42.30 |
51.32 |
9.02 |
|
Mitchell, Vincent P (10)
|
AMANSC |
41.53 |
DQ |
- |
|
Hollenbeck, Caden J (8)
|
AMANSC |
41.55 |
NS |
- |
|
Morrell, Andrew M (10)
|
AMANSC |
39.00 |
NS |
- |
|
Morgan, Tabari A (10)
|
AMRESY |
41.80 |
NS |
- |