| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Caulkins, Lila M (9)
|
AMNGAC |
23.13 |
23.07 |
-0.06 |
| 2 |
Werner, Ayla R (10)
|
AMNHA |
26.36 |
24.40 |
-1.96 |
| 3 |
Cornell, Olivia R (10)
|
AMWAAC |
30.76 |
24.58 |
-6.18 |
| 4 |
Holloway, Carson G (9)
|
AMWAAC |
31.31 |
24.69 |
-6.62 |
| 5 |
Peace, Meela J (10)
|
AMCSC |
26.18 |
25.03 |
-1.15 |
| 6 |
Dickey, Brooke A (10)
|
AMNGAC |
31.59 |
25.32 |
-6.27 |
| 7 |
Caulkins, Grace M (9)
|
AMNGAC |
26.83 |
25.52 |
-1.31 |
| 8 |
Shellenberger, Clara P (9)
|
AMMLAC |
27.92 |
25.60 |
-2.32 |
| 9 |
Tedesco, Amelia M (9)
|
AMUN-K |
38.02 |
28.47 |
-9.55 |
| 10 |
Valenti, Rylee A (9)
|
AMCSC |
33.22 |
29.67 |
-3.55 |
| 11 |
Wheeler, Logan R (9)
|
AMHD |
34.85 |
32.89 |
-1.96 |
|
Bentz, Lena R (9)
|
AMNHA |
28.42 |
DQ |
- |
|
DeChicchis, Sabrina E (10)
|
AMPTSC |
40.20 |
DQ |
- |
|
Ressler, Nicola L (9)
|
AMKW |
49.02 |
NS |
- |