| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Puthoff, Mackenzie J (7)
|
AMMOON |
21.20 |
18.49 |
-2.71 |
| 2 |
Keeley, Emma M (7)
|
AMWAAC |
25.06 |
22.80 |
-2.26 |
| 3 |
Capp, Molly L (7)
|
AMMOON |
29.33 |
23.26 |
-6.07 |
| 4 |
Bittner, Mia A (7)
|
AMRXA |
26.97 |
24.15 |
-2.82 |
| 5 |
Cegan, Olivia R (7)
|
AMHD |
28.10 |
24.31 |
-3.79 |
| 6 |
Coffman, Margaret J (7)
|
AMANSC |
30.32 |
24.38 |
-5.94 |
| 7 |
Ressler, Shyloh W (7)
|
AMPRA |
36.87 |
27.00 |
-9.87 |
| 8 |
Alexander, Annabelle E (7)
|
AMSVSC |
26.72 |
28.00 |
1.28 |
| 9 |
Phipps, Cecilia A (7)
|
AMHD |
32.98 |
28.30 |
-4.68 |
| 10 |
Radomski, Annabelle M (7)
|
AMHD |
38.98 |
28.80 |
-10.18 |
| 11 |
Page, Brynley J (7)
|
AMHD |
49.98 |
29.50 |
-20.48 |
| 12 |
Liddle, Tess G (7)
|
AMPTSC |
30.20 |
29.62 |
-0.58 |
| 13 |
Ward, Ariana R (7)
|
AMWAAC |
30.86 |
29.95 |
-0.91 |
| 14 |
Robinson, Gabrielle D (7)
|
AMSFAC |
38.19 |
32.04 |
-6.15 |
| 15 |
Cornell, Elyse M (7)
|
AMWAAC |
54.69 |
38.09 |
-16.60 |
| 16 |
Berke, Mikayla R (7)
|
AMHD |
43.98 |
41.43 |
-2.55 |
|
Yoezle, Gia R (7)
|
AMSFAC |
33.19 |
NS |
- |
|
Pipes, Kayley G (7)
|
AMWAAC |
30.25 |
NS |
- |
|
Hollenbeck, Mackenzie E (7)
|
AMANSC |
21.79 |
NS |
- |