| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Roncone, Aria R (7)
|
AMSFAC |
46.64 |
47.70 |
1.06 |
| 2 |
Troiana, Riya I (7)
|
AMHD |
52.36 |
52.73 |
0.37 |
| 3 |
Capp, Molly L (7)
|
AMMOON |
1:01.12 |
54.68 |
-6.44 |
| 4 |
Coffman, Margaret J (7)
|
AMANSC |
1:02.43 |
55.58 |
-6.85 |
| 5 |
Cegan, Olivia R (7)
|
AMHD |
1:00.70 |
58.37 |
-2.33 |
| 6 |
Puthoff, Mackenzie J (7)
|
AMMOON |
56.94 |
58.53 |
1.59 |
| 7 |
Alexander, Annabelle E (7)
|
AMSVSC |
1:02.72 |
1:06.46 |
3.74 |
| 8 |
Bittner, Mia A (7)
|
AMRXA |
1:07.50 |
1:07.14 |
-0.36 |
| 9 |
Liddle, Tess G (7)
|
AMPTSC |
1:20.20 |
1:11.85 |
-8.35 |
| 10 |
Cornell, Elyse M (7)
|
AMWAAC |
1:37.33 |
1:12.97 |
-24.36 |
| 11 |
Page, Brynley J (7)
|
AMHD |
1:40.98 |
1:14.55 |
-26.43 |
| 12 |
Radomski, Annabelle M (7)
|
AMHD |
1:29.98 |
1:19.28 |
-10.70 |
| 13 |
Ressler, Shyloh W (7)
|
AMPRA |
1:34.47 |
1:25.67 |
-8.80 |
| 14 |
Berke, Mikayla R (7)
|
AMHD |
1:34.98 |
1:36.36 |
1.38 |
|
Phipps, Cecilia A (7)
|
AMHD |
1:19.98 |
DQ |
- |
|
Keeley, Emma M (7)
|
AMWAAC |
58.84 |
DQ |
- |
|
Yoezle, Gia R (7)
|
AMSFAC |
1:15.19 |
NS |
- |
|
Pipes, Kayley G (7)
|
AMWAAC |
1:01.43 |
NS |
- |