| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Capp, Molly L (7)
|
AMMOON |
48.12 |
30.03 |
-18.09 |
| 2 |
Puthoff, Mackenzie J (7)
|
AMMOON |
31.19 |
30.06 |
-1.13 |
| 3 |
Troiana, Riya I (7)
|
AMHD |
49.05 |
32.54 |
-16.51 |
| 4 |
Ward, Ariana R (7)
|
AMWAAC |
36.78 |
36.37 |
-0.41 |
| 5 |
Coffman, Margaret J (7)
|
AMANSC |
39.79 |
38.30 |
-1.49 |
| 6 |
Alexander, Annabelle E (7)
|
AMSVSC |
39.14 |
43.27 |
4.13 |
| 7 |
Cornell, Elyse M (7)
|
AMWAAC |
1:15.45 |
1:03.79 |
-11.66 |
|
Cegan, Olivia R (7)
|
AMHD |
45.98 |
DQ |
- |
|
Phipps, Cecilia A (7)
|
AMHD |
36.98 |
DQ |
- |
|
Liddle, Tess G (7)
|
AMPTSC |
42.20 |
DQ |
- |
|
Bittner, Mia A (7)
|
AMRXA |
39.15 |
DQ |
- |
|
Robinson, Gabrielle D (7)
|
AMSFAC |
40.19 |
DQ |
- |
|
Hollenbeck, Mackenzie E (7)
|
AMANSC |
27.31 |
NS |
- |
|
Yoezle, Gia R (7)
|
AMSFAC |
36.19 |
NS |
- |
|
Pipes, Kayley G (7)
|
AMWAAC |
50.80 |
NS |
- |