| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Juliano, Paige E (13)
|
AMUN-M |
3:10.17 |
2:53.66 |
-16.51 |
| 2 |
Hull, Marideth G (13)
|
AMUN |
3:03.09 |
2:56.10 |
-6.99 |
| 3 |
Lowman, Sarah N (13)
|
AMWAAC |
3:15.45 |
3:02.45 |
-13.00 |
| 4 |
Alexander, Gabrielle C (13)
|
AMSVSC |
3:08.93 |
3:06.54 |
-2.39 |
| 5 |
Rowland, Natalie E (13)
|
AMBWA |
3:37.16 |
3:21.95 |
-15.21 |
| 6 |
Abraham, Elizabeth S (14)
|
AMWAAC |
3:23.51 |
3:27.62 |
4.11 |
| 7 |
Zanella, Torri R (13)
|
AMWAAC |
3:33.87 |
3:30.92 |
-2.95 |
| 8 |
Droschak, Madison E (13)
|
AMSHAQ |
4:08.88 |
3:40.44 |
-28.44 |
|
Bhat, Divya (14)
|
AMSFAC |
3:21.80 |
NS |
- |
|
Katta, Triyasree (14)
|
AMSFAC |
2:58.53 |
NS |
- |