| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Han, Katherine (7)
|
AMKW |
1:09.02 |
23.69 |
-45.33 |
| 2 |
Puthoff, Mackenzie J (7)
|
AMMOON |
29.09 |
25.56 |
-3.53 |
| 3 |
Capp, Molly L (7)
|
AMMOON |
27.83 |
27.03 |
-0.80 |
| 4 |
Coffman, Margaret J (7)
|
AMANSC |
35.75 |
27.06 |
-8.69 |
| 5 |
Jubeck, Claire J (7)
|
AMMOON |
46.18 |
28.11 |
-18.07 |
| 6 |
Cegan, Olivia R (7)
|
AMHD |
44.21 |
30.00 |
-14.21 |
| 7 |
Alexander, Annabelle E (7)
|
AMSVSC |
27.07 |
30.03 |
2.96 |
| 8 |
Bittner, Mia A (7)
|
AMRXA |
33.13 |
30.15 |
-2.98 |
| 9 |
Cornell, Elyse M (7)
|
AMWAAC |
36.02 |
31.49 |
-4.53 |
| 10 |
Robinson, Gabrielle D (7)
|
AMSFAC |
39.19 |
34.06 |
-5.13 |
| 11 |
Ward, Ariana R (7)
|
AMWAAC |
33.48 |
35.38 |
1.90 |
| 12 |
Page, Brynley J (7)
|
AMHD |
48.98 |
35.47 |
-13.51 |
| 13 |
Radomski, Annabelle M (7)
|
AMHD |
42.98 |
36.95 |
-6.03 |
| 14 |
Berke, Mikayla R (7)
|
AMHD |
45.98 |
44.71 |
-1.27 |
|
Ressler, Shyloh W (7)
|
AMPRA |
38.47 |
DQ |
- |
|
Oliver, Emma E (7)
|
AMKW |
45.02 |
DQ |
- |
|
Yoezle, Gia R (7)
|
AMSFAC |
36.19 |
NS |
- |
|
Hollenbeck, Mackenzie E (7)
|
AMANSC |
27.55 |
NS |
- |