| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hunsinger, Reese E (8)
|
AMFAST |
26.52 |
20.57 |
-5.95 |
| 2 |
Donaldson, Ava J (8)
|
AMANSC |
32.72 |
22.83 |
-9.89 |
| 3 |
Powell, Abigail C (8)
|
AMANSC |
30.00 |
22.95 |
-7.05 |
| 4 |
Ukrayinets, Sofiya (8)
|
AMANSC |
32.72 |
23.00 |
-9.72 |
| 5 |
Sunderlin, Charlotte R (8)
|
AMANSC |
33.73 |
25.97 |
-7.76 |
| 6 |
Miller, Cora J (8)
|
AMANSC |
30.00 |
29.42 |
-0.58 |
| 7 |
Rice, Celinda M (8)
|
AMGPAC |
29.20 |
29.85 |
0.65 |
| 8 |
Emekter, Selin E (8)
|
AMUN-A |
33.73 |
30.30 |
-3.43 |
|
Constable, Aubrey C (8)
|
AMANSC |
31.71 |
DQ |
- |
|
Ukrayinets, Anastasiya (8)
|
AMANSC |
34.74 |
DQ |
- |
|
DeCaria, Lisa A (8)
|
AMMOON |
49.85 |
DQ |
- |
|
Marks, Ariana R (8)
|
AMSVSC |
30.92 |
DQ |
- |