| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Opitz, Lillian K (7)
|
AMPEAQ |
52.22 |
49.20 |
-3.02 |
| 2 |
Turner, Alyssa J (7)
|
AMRACE |
51.11 |
53.05 |
1.94 |
| 3 |
Drozd, Emily M (7)
|
AMUN-A |
1:08.99 |
56.06 |
-12.93 |
| 4 |
Hull, Naomi D (7)
|
AMFAST |
1:04.36 |
58.66 |
-5.70 |
| 5 |
Alexander, Annabelle E (7)
|
AMSVSC |
1:14.47 |
1:02.72 |
-11.75 |
| 6 |
Coffman, Margaret J (7)
|
AMANSC |
1:02.43 |
1:03.56 |
1.13 |
| 7 |
Hollenbeck, Mackenzie E (7)
|
AMANSC |
1:01.69 |
1:03.84 |
2.15 |
| 8 |
Ponder, Alacia M (7)
|
AMRACE |
1:27.00 |
1:06.00 |
-21.00 |
| 9 |
Bittner, Mia A (7)
|
AMRXA |
1:30.99 |
1:08.24 |
-22.75 |
| 10 |
Hunsinger, Sadie G (7)
|
AMFAST |
1:15.57 |
1:11.55 |
-4.02 |
| 11 |
Proie, Alexandra S (7)
|
AMPRA |
1:49.47 |
1:15.71 |
-33.76 |
|
Puthoff, Mackenzie J (7)
|
AMMOON |
59.63 |
DQ |
- |