| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Opitz, Lillian K (7)
|
AMPEAQ |
46.87 |
43.02 |
-3.85 |
| 2 |
Turner, Alyssa J (7)
|
AMRACE |
46.63 |
45.74 |
-0.89 |
| 3 |
Hollenbeck, Mackenzie E (7)
|
AMANSC |
52.98 |
53.02 |
0.04 |
| 4 |
Drozd, Emily M (7)
|
AMUN-A |
58.31 |
53.14 |
-5.17 |
| 5 |
Murphy, Olivia C (7)
|
AMBWA |
1:25.16 |
57.67 |
-27.49 |
| 6 |
Alexander, Annabelle E (7)
|
AMSVSC |
1:10.48 |
59.39 |
-11.09 |
| 7 |
Hunsinger, Sadie G (7)
|
AMFAST |
1:09.55 |
59.49 |
-10.06 |
| 8 |
Proie, Alexandra S (7)
|
AMPRA |
1:37.47 |
1:03.23 |
-34.24 |
| 9 |
Bittner, Mia A (7)
|
AMRXA |
1:28.99 |
1:04.34 |
-24.65 |
| 10 |
Ponder, Alacia M (7)
|
AMRACE |
1:29.14 |
1:19.17 |
-9.97 |
| 11 |
Palkovitch, Scarlet M (7)
|
AMRACE |
1:23.14 |
1:29.13 |
5.99 |