| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kleiman, Grace D (10)
|
GUKATY |
47.07 |
47.02 |
-0.05 |
| 2 |
Capehart, Madison (9)
|
GUAGS |
50.22 |
48.04 |
-2.18 |
| 3 |
McKown, Mallory A (9)
|
GUPACK |
49.00 |
49.08 |
0.08 |
| 4 |
Richey, Madeline K (10)
|
GUAMBU |
55.86 |
50.95 |
-4.91 |
| 5 |
Duerr, Sadie G (10)
|
GUMAC |
52.52 |
51.57 |
-0.95 |
| 6 |
Stofberg, Kayla N (9)
|
GUKATY |
52.57 |
51.66 |
-0.91 |
| 7 |
Sosa, Anna M (10)
|
GUMAC |
54.78 |
54.33 |
-0.45 |
| 8 |
Leonard, Kilynn N (10)
|
GUMAC |
55.36 |
55.83 |
0.47 |
| 9 |
Schueder, Emma J (10)
|
GUSSAN |
55.02 |
57.45 |
2.43 |
| 10 |
Quave, Juliette F (9)
|
GUAGS |
1:02.38 |
59.86 |
-2.52 |
| 11 |
Leonard, Kinley E (9)
|
GUMAC |
1:06.94 |
1:00.54 |
-6.40 |
| 12 |
Nguyen, Alanna H (9)
|
GULSST |
1:04.35 |
1:02.15 |
-2.20 |
| 13 |
Ammons, Natalie G (10)
|
GUPACE |
1:05.09 |
1:04.43 |
-0.66 |
| 14 |
Zepeda-Otalora, Camila J (9)
|
GUKATY |
1:13.04 |
1:05.21 |
-7.83 |
| 15 |
Gallagher, Anabelle G (9)
|
GUMAC |
|
1:07.01 |
- |
| 16 |
Nelson, Brooklyn C (9)
|
GUKATY |
1:05.36 |
1:08.25 |
2.89 |
| 17 |
Patterson, Emma M (10)
|
GUPACK |
1:10.61 |
1:08.30 |
-2.31 |
|
Lopez DE LA Rosa, Luna N (9)
|
GUKATY |
|
DQ |
- |
|
McGregor, Moira W (9)
|
GUSHRK |
57.54 |
DQ |
- |