| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Aragon Calzada, Luciana (8)
|
GUMAC |
47.85 |
45.58 |
-2.27 |
| 2 |
Yang, Chloe Y (7)
|
GUSSAN |
44.64 |
46.11 |
1.47 |
| 3 |
Stidham, Brooklyn E (7)
|
GUAMBU |
46.80 |
48.09 |
1.29 |
| 4 |
Masch, Bianca D (8)
|
GUKATY |
46.90 |
48.90 |
2.00 |
| 5 |
Dumitrascu, Sofie (8)
|
GUKATY |
54.41 |
50.40 |
-4.01 |
| 6 |
Oquendo, Jorlaney M (8)
|
GUPACK |
53.19 |
51.27 |
-1.92 |
| 7 |
Bhakta, Aniya K (8)
|
GUSHRK |
50.99 |
51.45 |
0.46 |
| 8 |
McDaniel, Kenley E (8)
|
GUMAC |
49.52 |
51.54 |
2.02 |
| 9 |
Yang, Vivian X (8)
|
GUKATY |
54.72 |
52.08 |
-2.64 |
| 10 |
Botens, Phoebe H (8)
|
GUKATY |
1:07.63 |
54.53 |
-13.10 |
| 11 |
Judd, Veronica G (8)
|
GUKATY |
46.78 |
54.72 |
7.94 |
| 12 |
Treadaway, Caeleigh R (8)
|
GUMAC |
53.83 |
55.22 |
1.39 |
| 13 |
Griffin-Coyle, Addyson G (7)
|
GUMAC |
58.14 |
57.10 |
-1.04 |
| 14 |
Moore, Kivlyn A (6)
|
GUSHRK |
57.76 |
58.79 |
1.03 |
| 15 |
Xiang, Maggie N (7)
|
GUKATY |
58.34 |
1:00.88 |
2.54 |
| 16 |
Reams, Piper J (8)
|
GUPACE |
1:04.68 |
1:02.65 |
-2.03 |
| 17 |
Skinner, Sarah E (8)
|
GUMAC |
58.26 |
1:03.78 |
5.52 |
| 18 |
Zhang, Elizabeth C (6)
|
GUKATY |
|
1:04.08 |
- |
| 19 |
Jessop, Lenet R (7)
|
GULSST |
58.65 |
1:05.37 |
6.72 |