| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McKown, Mallory A (9)
|
GUPACK |
44.19 |
44.94 |
0.75 |
| 2 |
Dickamore, Kathrin I (9)
|
GUSHRK |
47.62 |
45.50 |
-2.12 |
| 3 |
Kleiman, Grace D (10)
|
GUKATY |
48.68 |
46.06 |
-2.62 |
| 4 |
Duerr, Sadie G (10)
|
GUMAC |
42.14 |
46.11 |
3.97 |
| 5 |
Leonard, Kinley E (9)
|
GUMAC |
50.52 |
47.97 |
-2.55 |
| 6 |
Schueder, Emma J (10)
|
GUSSAN |
47.49 |
48.92 |
1.43 |
| 7 |
Sosa, Anna M (10)
|
GUMAC |
47.54 |
48.98 |
1.44 |
| 8 |
Leonard, Kilynn N (10)
|
GUMAC |
46.05 |
49.39 |
3.34 |
| 9 |
McGregor, Moira W (9)
|
GUSHRK |
50.14 |
50.65 |
0.51 |
| 10 |
Quave, Juliette F (9)
|
GUAGS |
51.09 |
54.53 |
3.44 |
| 11 |
Zepeda-Otalora, Camila J (9)
|
GUKATY |
55.11 |
55.96 |
0.85 |
| 12 |
Duong, Trinity T (9)
|
GUPACK |
|
59.72 |
- |
| 13 |
Lopez DE LA Rosa, Luna N (9)
|
GUKATY |
1:05.46 |
1:01.06 |
-4.40 |
| 14 |
Gallagher, Anabelle G (9)
|
GUMAC |
|
1:01.25 |
- |
| 15 |
Jacobson, Katherine R (9)
|
GUSHRK |
1:14.38 |
1:03.32 |
-11.06 |