| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Finanger, Kaia E (8)
|
GUVSST |
40.91 |
41.76 |
0.85 |
| 2 |
Duncan, Lani P (8)
|
GUCATS |
43.53 |
43.39 |
-0.14 |
| 3 |
Chen, Mei Ning (8)
|
GUVSST |
50.59 |
47.62 |
-2.97 |
| 4 |
Meyer, Alice L (8)
|
GUVSST |
46.57 |
49.48 |
2.91 |
| 5 |
Vann, McKenna J (8)
|
GUFCST |
52.48 |
49.71 |
-2.77 |
| 6 |
Jiao, Olivia H (8)
|
GUFCST |
|
52.39 |
- |
| 7 |
Karim, Sarah (8)
|
GUFCST |
56.28 |
55.42 |
-0.86 |
| 8 |
Chen, Mackenzie Y (8)
|
GUFCST |
56.04 |
1:00.13 |
4.09 |
| 9 |
Blackmon, Kyra L (8)
|
GUAMBU |
1:08.58 |
1:06.81 |
-1.77 |
| 10 |
Nguyen, Chloe E (8)
|
GUFCST |
1:07.81 |
1:06.90 |
-0.91 |
|
Duculan, Johnery Kaye S (8)
|
GUFCST |
|
DQ |
- |