| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lundquist, Rune A (11)
|
GUSWIM |
35.94 |
35.01 |
-0.93 |
| 2 |
Schauss, William T (12)
|
GUAQUA |
37.87 |
35.63 |
-2.24 |
| 3 |
Haberman, Hunter H (11)
|
GUTWST |
35.78 |
37.00 |
1.22 |
| 4 |
DelaCruz, Jacob E (11)
|
GUCFSC |
38.07 |
37.58 |
-0.49 |
| 5 |
Ho, Brian A (11)
|
GUCFSC |
40.36 |
39.30 |
-1.06 |
| 6 |
Wu, Andrew (12)
|
GUCFSC |
42.18 |
39.69 |
-2.49 |
| 7 |
McAdory, Dustin H (12)
|
GUTWST |
42.70 |
39.77 |
-2.93 |
| 8 |
Morales, Alexzander X (12)
|
GUAQUA |
42.61 |
40.18 |
-2.43 |
| 9 |
Cavazos, Joaquin G (11)
|
GUCFSC |
44.57 |
40.73 |
-3.84 |
| 10 |
Knight, Theodore III R (11)
|
GUAQUA |
39.82 |
40.98 |
1.16 |
| 11 |
Kurka, George J (11)
|
GUSHAC |
38.57 |
41.35 |
2.78 |
| 12 |
Samuels, Ethan L (11)
|
GUPACK |
40.06 |
42.39 |
2.33 |
| 13 |
LeBlanc, Luca D (11)
|
GUPACK |
52.70 |
43.79 |
-8.91 |
| 14 |
Foster, Tristan R (11)
|
GUPACK |
|
44.96 |
- |
| 15 |
Wall, Aiden J (12)
|
GUPACK |
51.15 |
46.51 |
-4.64 |
| 16 |
Jessop, Maric K (11)
|
GULSST |
49.87 |
47.60 |
-2.27 |
| 17 |
Rodriguez, Jacob A (11)
|
GUBATS |
46.87 |
47.75 |
0.88 |
| 18 |
Mowles, Jaren A (12)
|
GUBATS |
|
49.11 |
- |
| 19 |
Yacoub, Brady E (11)
|
GUTWST |
50.51 |
49.99 |
-0.52 |
|
Rains, Logan J (11)
|
GUPACK |
|
DQ |
- |
|
Smith, Parker E (11)
|
GUCFSC |
41.63 |
NS |
- |