| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zhao, Johnathan M (11)
|
GUPACK |
30.48 |
28.94 |
-1.54 |
| 2 |
Samuels, Ethan L (11)
|
GUPACK |
31.17 |
30.23 |
-0.94 |
| 3 |
Schauss, William T (12)
|
GUAQUA |
32.39 |
31.39 |
-1.00 |
| 4 |
Chen, Ziqian (12)
|
GUTWST |
32.82 |
32.26 |
-0.56 |
| 5 |
Hedlund, Nathan C (11)
|
GUPACK |
33.27 |
32.37 |
-0.90 |
| 6 |
Kurka, George J (11)
|
GUSHAC |
31.88 |
32.92 |
1.04 |
| 7 |
Wu, Andrew (12)
|
GUCFSC |
35.19 |
32.95 |
-2.24 |
| 8 |
Lundquist, Rune A (11)
|
GUSWIM |
32.08 |
33.09 |
1.01 |
| 9 |
Morales, Alexzander X (12)
|
GUAQUA |
39.45 |
33.15 |
-6.30 |
| 10 |
Knight, Theodore III R (11)
|
GUAQUA |
33.13 |
33.22 |
0.09 |
| 11 |
DelaCruz, Jacob E (11)
|
GUCFSC |
33.24 |
33.61 |
0.37 |
| 12 |
McAdory, Dustin H (12)
|
GUTWST |
36.21 |
33.73 |
-2.48 |
| 13 |
Wall, Aiden J (12)
|
GUPACK |
34.16 |
35.67 |
1.51 |
| 14 |
Rodriguez, Jacob A (11)
|
GUBATS |
38.45 |
36.55 |
-1.90 |
| 15 |
Denton, Owen T (11)
|
GUPACK |
37.01 |
37.34 |
0.33 |
| 16 |
Foster, Tristan R (11)
|
GUPACK |
|
39.78 |
- |
| 17 |
Jessop, Maric K (11)
|
GULSST |
45.51 |
40.45 |
-5.06 |
| 18 |
Prejean, Conner M (12)
|
GUSHAC |
46.02 |
40.78 |
-5.24 |
|
Ho, Brian A (11)
|
GUCFSC |
33.01 |
NS |
- |
|
Cavazos, Joaquin G (11)
|
GUCFSC |
36.79 |
NS |
- |