| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Finanger, Kaia E (8)
|
GUTWST |
55.22 |
44.19 |
-11.03 |
| 2 |
Schauss, Ingrid A (8)
|
GUAQUA |
49.81 |
51.54 |
1.73 |
| 3 |
Horne, Sarah L (8)
|
GUPACK |
53.86 |
52.60 |
-1.26 |
| 4 |
Sanchez, Adelle P (8)
|
GUTWST |
56.03 |
53.03 |
-3.00 |
| 5 |
Chen, Mei Ning (8)
|
GUVSST |
54.08 |
53.09 |
-0.99 |
| 6 |
Shen, Arianna L (8)
|
GUCFSC |
1:00.20 |
54.01 |
-6.19 |
| 7 |
Wiles, Sydney C (8)
|
GUTWST |
59.87 |
54.09 |
-5.78 |
| 8 |
Wheeler, Delaney R (7)
|
GUCFSC |
59.93 |
56.44 |
-3.49 |
| 9 |
Bajaj, Aryla (8)
|
GUTWST |
1:00.34 |
57.10 |
-3.24 |
| 10 |
D'Anna, Emma L (8)
|
GUCFSC |
1:02.96 |
57.35 |
-5.61 |
| 11 |
Oquendo, Jorlaney M (8)
|
GUPACK |
59.48 |
58.25 |
-1.23 |
| 12 |
Hsieh, Kaitlyn M (8)
|
GUPACK |
56.33 |
58.29 |
1.96 |
| 13 |
Shan, Sophie W (8)
|
GUCFSC |
|
59.18 |
- |
| 14 |
Pacoma, Kaylee Angela D (7)
|
GUCFSC |
1:05.97 |
59.73 |
-6.24 |
| 15 |
Rosas Magos, Andrea (8)
|
GUTWST |
1:19.97 |
1:00.56 |
-19.41 |
| 16 |
Milano, Caroline A (7)
|
GUCFSC |
1:06.19 |
1:01.20 |
-4.99 |
| 17 |
Betts, Ava G (8)
|
GUCFSC |
1:06.49 |
1:01.83 |
-4.66 |
| 18 |
Heredia, Amanda (8)
|
GUCFSC |
1:22.06 |
1:02.05 |
-20.01 |
| 19 |
Chen, Yan Ning (6)
|
GUVSST |
1:06.94 |
1:03.28 |
-3.66 |
| 20 |
Wright, Mia M (8)
|
GUPACK |
1:07.94 |
1:04.19 |
-3.75 |
| 21 |
Goebel, Danika R (8)
|
GUPACK |
1:05.82 |
1:05.23 |
-0.59 |
| 22 |
Espinosa-Agrait, Juliana I (6)
|
GUTWST |
1:29.36 |
1:07.45 |
-21.91 |
| 23 |
Nasr, Isabella Y (7)
|
GUCFSC |
1:17.63 |
1:14.27 |
-3.36 |
| 24 |
Vaughan, Layla L (8)
|
GUPACK |
1:13.17 |
1:15.03 |
1.86 |
| 25 |
Svoboda, Maryanne C (6)
|
GUCFSC |
1:14.69 |
1:15.92 |
1.23 |
| 26 |
Jessop, Lenet R (7)
|
GULSST |
1:20.30 |
1:16.14 |
-4.16 |
| 27 |
Olson, Ingrid E (7)
|
GUCFSC |
1:27.66 |
1:16.73 |
-10.93 |
|
Davis, Audrey E (8)
|
GUTWST |
|
DQ |
- |
|
Chong, Jillian T (7)
|
GULSST |
1:33.99 |
DQ |
- |
|
Marceleno, Sofia (8)
|
GUHCAP |
1:06.14 |
DQ |
- |