| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stoup, Anna L (12)
|
ADCPP |
3:30.51 |
3:06.12 |
-24.39 |
| 2 |
Antolick, Caroline B (12)
|
ADALBS |
3:22.75 |
3:07.69 |
-15.06 |
| 3 |
Kaufman, Ava R (12)
|
ADDELM |
2:44.08 |
3:08.61 |
24.53 |
| 4 |
Goodspeed, Fayth R (11)
|
ADGLEN |
3:15.55 |
3:13.56 |
-1.99 |
| 5 |
Sellnow, Emma M (12)
|
ADEELS |
3:19.45 |
3:14.28 |
-5.17 |
| 6 |
Rochefort, Sydney E (12)
|
ADNTS |
3:28.90 |
3:14.96 |
-13.94 |
| 7 |
Zhang, Stella Y (12)
|
ADCPP |
3:20.36 |
3:20.37 |
0.01 |
| 8 |
Spina, Sophie E (12)
|
ADCLIN |
3:20.54 |
3:22.45 |
1.91 |
| 9 |
Schloth, Katherine I (12)
|
ADUN |
3:35.77 |
3:25.42 |
-10.35 |
| 10 |
Cho, Eileen Y (12)
|
ADALBS |
2:56.18 |
3:25.88 |
29.70 |
| 11 |
Lind, Samantha P (12)
|
ADALBS |
3:06.96 |
3:34.89 |
27.93 |