| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Braun, Gretchen L (11)
|
ADNTS |
5:07.70 |
4:59.81 |
-7.89 |
| 2 |
Stoup, Anna L (12)
|
ADCPP |
5:43.04 |
5:06.70 |
-36.34 |
| 3 |
Kaufman, Ava R (12)
|
ADDELM |
5:35.70 |
5:11.33 |
-24.37 |
| 4 |
Boenau, Carlye Ana T (12)
|
ADBFIN |
5:16.57 |
5:18.76 |
2.19 |
| 5 |
Bieniek, Allison M (12)
|
ADCPP |
5:36.83 |
5:19.84 |
-16.99 |
| 6 |
O'Donnell, Maura E (12)
|
ADEELS |
5:29.04 |
5:21.25 |
-7.79 |
| 7 |
Spina, Sophie E (12)
|
ADCLIN |
5:31.11 |
5:21.60 |
-9.51 |
| 8 |
Schloth, Katherine I (12)
|
ADUN |
5:52.16 |
5:23.72 |
-28.44 |
| 9 |
Michels, Delaney R (12)
|
ADCPP |
6:16.87 |
5:31.55 |
-45.32 |
| 10 |
Xue, Nichole Y (12)
|
ADCPP |
6:25.64 |
5:31.91 |
-53.73 |
| 11 |
Antolick, Caroline B (12)
|
ADALBS |
6:07.42 |
5:36.14 |
-31.28 |
| 12 |
Harpootlian, Hannah R (11)
|
ADCPP |
6:35.86 |
5:53.63 |
-42.23 |
| 13 |
Tolan, Sydney T (12)
|
ADSCHE |
5:55.04 |
5:58.39 |
3.35 |