| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rudd, Conner D (12)
|
ADCPP |
2:41.44 |
2:41.59 |
0.15 |
| 2 |
Brkanovic, Isak A (12)
|
ADDELM |
2:53.33 |
2:45.10 |
-8.23 |
| 3 |
Durr, Tucker C (12)
|
ADSCHE |
2:48.15 |
2:46.65 |
-1.50 |
| 4 |
Wettig, Gabriel J (12)
|
ADSCHE |
2:56.54 |
2:49.50 |
-7.04 |
| 5 |
Hershberg, Jacob C (11)
|
ADDELM |
3:00.33 |
2:51.29 |
-9.04 |
| 6 |
Gregory, John D (11)
|
ADSCHE |
2:54.42 |
2:52.69 |
-1.73 |
| 7 |
Di Fabio, Luka D (11)
|
ADSCHE |
3:01.87 |
2:53.37 |
-8.50 |
| 8 |
O'Leary, Anthony Y (11)
|
ADSCHE |
3:07.93 |
3:01.09 |
-6.84 |
| 9 |
Tieckelmann, Connor R (12)
|
ADCPP |
3:04.83 |
3:01.24 |
-3.59 |
| 10 |
Karaca, Rowan H (12)
|
ADSCHE |
3:10.31 |
3:01.44 |
-8.87 |
| 11 |
Wilkosz, Matthew M (12)
|
ADCLIN |
3:14.81 |
3:07.13 |
-7.68 |
| 12 |
Wang, Maxwell C (12)
|
ADSCHE |
2:47.86 |
3:08.55 |
20.69 |
| 13 |
Cao, James Y (11)
|
ADDELM |
3:28.28 |
3:10.37 |
-17.91 |
| 14 |
Mosaad, Karim M (11)
|
ADNHA |
3:12.23 |
3:11.78 |
-0.45 |
| 15 |
McNair, John P (11)
|
ADCLIN |
3:16.99 |
3:12.47 |
-4.52 |
| 16 |
Reichl, Dylan J (12)
|
ADBFIN |
3:14.48 |
3:15.32 |
0.84 |
| 17 |
Hook, Gavin M (12)
|
ADCPP |
3:16.80 |
3:16.79 |
-0.01 |
| 18 |
Zhang, Alexander W (11)
|
ADSCHE |
3:20.02 |
3:17.06 |
-2.96 |
| 19 |
Zeliph, Thomas J (11)
|
ADALBS |
3:21.85 |
3:19.81 |
-2.04 |
| 20 |
Staudinger, Samuel P (11)
|
ADALBS |
3:17.92 |
3:23.45 |
5.53 |
| 21 |
Lischinsky, Stephen J (12)
|
ADHAWK |
3:40.28 |
3:29.22 |
-11.06 |
| 22 |
Bhave, Advait G (12)
|
ADCPP |
3:38.84 |
3:32.04 |
-6.80 |
| 23 |
McCoy, William G (12)
|
ADCPP |
3:46.51 |
3:40.20 |
-6.31 |
| 24 |
Mensing, Bryce W (11)
|
ADEELS |
3:54.50 |
3:41.10 |
-13.40 |
|
Hellenthal, Thomas M (11)
|
ADCLIN |
2:44.01 |
DQ |
- |
|
Killian, Daniel S (12)
|
ADCPP |
3:10.10 |
DQ |
- |