| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Drewery, Laura E (11)
|
GUAMBU |
44.19 |
39.68 |
-4.51 |
| 2 |
Austin, Gabrielle A (12)
|
GUSPA |
45.67 |
39.97 |
-5.70 |
| 3 |
Isaac, Jessie (11)
|
GUPLAT |
38.50 |
41.39 |
2.89 |
| 4 |
Wood, Hadley K (12)
|
GUAGS |
43.11 |
43.07 |
-0.04 |
| 5 |
Riddle, Gloria (11)
|
GUAGS |
44.97 |
43.94 |
-1.03 |
| 6 |
Mendez Borges, Isabela H (11)
|
GURICE |
|
46.00 |
- |
| 7 |
Plowman, Alexandra M (11)
|
GUAMBU |
48.35 |
47.07 |
-1.28 |
| 8 |
Lopez DE LA Rosa, Victoria (11)
|
GUKATY |
49.61 |
51.54 |
1.93 |
| 9 |
Nielander, Maegan A (11)
|
GUKATY |
46.35 |
53.06 |
6.71 |
| 10 |
Molinaro, Liliana E (12)
|
GUPACK |
|
1:00.18 |
- |
|
Lopez, Jocelyne (11)
|
GUAMBU |
34.94 |
DQ |
- |