| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lowe, Dalton D (17)
|
GUSST |
48.06 |
51.98 |
3.92 |
| 2 |
Moser, Ashton J (17)
|
GULIFE |
53.86 |
54.11 |
0.25 |
| 3 |
Nace, Jackson E (15)
|
GUTYES |
57.43 |
55.08 |
-2.35 |
| 4 |
Weikel, Gabriel C (17)
|
GUESA |
53.31 |
55.86 |
2.55 |
| 5 |
Mora, Alex M (17)
|
GULIFE |
59.92 |
56.93 |
-2.99 |
| 6 |
Saria, Noah D (16)
|
GUTYES |
55.88 |
57.17 |
1.29 |
| 7 |
Prince, Toby B (15)
|
GUSST |
55.14 |
58.24 |
3.10 |
| 8 |
Armstrong, Jonathan W (44)
|
GULIFE |
57.80 |
58.33 |
0.53 |
| 9 |
Willetts, Benjamin C (16)
|
GUESA |
59.63 |
58.74 |
-0.89 |
| 10 |
Salgado, Nathan R (15)
|
GUTYES |
56.56 |
58.76 |
2.20 |
| 11 |
Diaz, Noah R (17)
|
GULIFE |
58.03 |
58.93 |
0.90 |
| 12 |
Murray, Aaron D (15)
|
GUSST |
59.02 |
59.06 |
0.04 |
| 13 |
Davis, Caleb M (15)
|
GUSST |
58.55 |
59.38 |
0.83 |
| 14 |
Granack, Samuel S (17)
|
GUSST |
|
59.55 |
- |
| 15 |
Armstrong, David J (16)
|
GULIFE |
55.98 |
1:00.14 |
4.16 |
| 16 |
Wood, Mason C (18)
|
GUAMBU |
|
1:00.84 |
- |
| 17 |
Nguyen, Johnathon L (15)
|
GUUN-T |
|
1:01.31 |
- |
| 18 |
Oster, Davis A (16)
|
NTTRAC |
58.16 |
1:01.65 |
3.49 |
| 19 |
Wheeler, Sean T (18)
|
GUSST |
|
1:02.23 |
- |
| 20 |
Nelson, Adam T (16)
|
GUSST |
|
1:03.95 |
- |
| 21 |
Eckert, Samuel F (15)
|
NTTRAC |
|
1:04.56 |
- |
| 22 |
Cicman, Logan J (16)
|
NTMTRO |
1:03.38 |
1:04.74 |
1.36 |
| 23 |
Parmelee, Carson D (18)
|
NTMTRO |
|
1:05.47 |
- |
| 24 |
Wylie, Bryson L (15)
|
GUSWIM |
1:02.33 |
1:06.10 |
3.77 |
| 25 |
De Leon, Arturo A (15)
|
GUESA |
|
1:10.98 |
- |
| 26 |
Blackshire, Clayton W (16)
|
GUAMBU |
1:08.65 |
1:12.62 |
3.97 |
| 27 |
Scott, Matthew D (15)
|
NTMTRO |
1:27.64 |
1:15.72 |
-11.92 |
| 28 |
Risner, Alton R (16)
|
GUSWIM |
|
1:16.54 |
- |