| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kolb, Diana E (17)
|
GUAMBU |
58.90 |
57.09 |
-1.81 |
| 2 |
Fortier, Katie E (16)
|
NTMTRO |
1:05.20 |
1:09.31 |
4.11 |
| 3 |
Davenport, Genna L (15)
|
GUSWIM |
|
1:13.46 |
- |
| 4 |
Isra, Lana E (15)
|
GUTYES |
1:15.20 |
1:13.54 |
-1.66 |
| 5 |
Vander Neut, Reilly A (15)
|
GUSWIM |
1:15.53 |
1:16.78 |
1.25 |
| 6 |
Cook, Rachel E (15)
|
GUTYES |
1:15.09 |
1:17.06 |
1.97 |
| 7 |
Flowers, Fatima (17)
|
GUAMBU |
1:17.34 |
1:19.26 |
1.92 |
| 8 |
Hoffman, Mackenzie L (16)
|
GUSWIM |
1:17.39 |
1:21.61 |
4.22 |
| 9 |
Fredregill, Sky B (17)
|
GUAMBU |
1:17.72 |
1:24.25 |
6.53 |
|
Ballin, Raven E (16)
|
GULIFE |
|
DQ |
- |
|
Stern, Maia E (15)
|
GUSST |
1:08.65 |
NS |
- |