| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Overholt, Sophia A (13)
|
NCYOTA |
36.88 |
36.66 |
-0.22 |
| 2 |
Eguiarte, Samara A (14)
|
NCYOTA |
38.03 |
38.80 |
0.77 |
| 3 |
Soroka, Emmery B (13)
|
NCYOTA |
38.48 |
38.99 |
0.51 |
| 4 |
Reich, Sophia G (13)
|
NCYOTA |
41.45 |
39.78 |
-1.67 |
| 5 |
Kovalaske, Katie A (13)
|
NCYOTA |
41.09 |
39.89 |
-1.20 |
| 6 |
Robertson, Isla E (13)
|
NCYOTA |
39.96 |
39.93 |
-0.03 |
| 7 |
Shin, Emma (13)
|
NCYOTA |
40.13 |
39.98 |
-0.15 |
| 8 |
Tirumanadham, Indira (13)
|
NCYOTA |
39.42 |
40.39 |
0.97 |
| 9 |
Organ, Zoe E (14)
|
NCYOTA |
|
40.47 |
- |
| 10 |
Stewart, Lola M (13)
|
NCYOTA |
44.97 |
40.73 |
-4.24 |
| 11 |
Smith, Jillian S (13)
|
NCYOTA |
44.32 |
42.76 |
-1.56 |
| 12 |
Peterson, Maya J (14)
|
NCYOTA |
43.38 |
42.88 |
-0.50 |
| 13 |
Macha, Esha (13)
|
NCYOTA |
45.74 |
44.16 |
-1.58 |
| 14 |
Shaffer, Abigail N (14)
|
NCYOTA |
|
45.32 |
- |
| 15 |
Schumacher, Amelia B (13)
|
NCYOTA |
48.77 |
45.87 |
-2.90 |
| 16 |
Quackenbush, Laycie M (14)
|
NCYOTA |
|
46.35 |
- |
|
Motteler, Abigail G (13)
|
NCYOTA |
41.21 |
DQ |
- |
|
Shalva, Michaela S (13)
|
NCYOTA |
37.07 |
NS |
- |