| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gladunin, Oleksandr (11)
|
MICW |
2:46.88 |
2:34.72 |
-12.16 |
| 2 |
Stanislawski, Jacob T (12)
|
MISST |
2:47.71 |
2:40.92 |
-6.79 |
| 3 |
Peters, Cole E (11)
|
OHGTAC |
2:36.87 |
2:41.23 |
4.36 |
| 4 |
Heberlein, Alexander G (11)
|
MICW |
2:46.40 |
2:47.61 |
1.21 |
| 5 |
Boutell, Vaughn S (12)
|
MICW |
2:57.23 |
2:48.05 |
-9.18 |
| 6 |
Corbett, Kyle G (11)
|
MIDASA |
3:00.00 |
2:48.20 |
-11.80 |
| 7 |
Antone, Charles R (12)
|
MIOLY |
3:43.65 |
2:53.13 |
-50.52 |
| 8 |
Lathrop, Thomas G (11)
|
OHGTAC |
3:06.68 |
2:53.69 |
-12.99 |
| 9 |
Dillman, Tyler P (11)
|
MICW |
2:53.53 |
2:56.90 |
3.37 |
| 10 |
Waters Jr., Ja'Von R (11)
|
MIDASA |
2:59.52 |
2:57.77 |
-1.75 |
| 11 |
Darnell, Donovan B (11)
|
MISST |
3:04.25 |
2:59.05 |
-5.20 |
| 12 |
Ngo, Evan D (11)
|
MISST |
3:09.08 |
3:02.29 |
-6.79 |
| 13 |
Baccus, Jonas A (11)
|
OHGTAC |
3:09.92 |
3:02.59 |
-7.33 |
| 14 |
Tishkowski, Alexander N (11)
|
MICW |
3:19.19 |
3:08.71 |
-10.48 |
|
Ivanov, Yegor V (11)
|
MICW |
3:13.88 |
DQ |
- |
|
May, Braylen R (11)
|
MISST |
3:03.58 |
DQ |
- |
|
Barcelon, Jonah O (12)
|
MICW |
2:50.52 |
DQ |
- |