| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gray, Amaleah R (9)
|
GUBATS |
46.60 |
45.36 |
-1.24 |
| 2 |
Jensen, Sarah A (9)
|
GUESA |
|
50.27 |
- |
| 3 |
Glowacki, Madelyn E (9)
|
GUESA |
51.33 |
54.94 |
3.61 |
| 4 |
Liddle, Heidi M (9)
|
GUESA |
57.40 |
56.61 |
-0.79 |
| 5 |
Burns, Brooke F (9)
|
GUNEHA |
|
57.52 |
- |
| 6 |
San Miguel, Eliana G (9)
|
GUESA |
1:10.01 |
58.78 |
-11.23 |
| 7 |
Martin, Lauren M (9)
|
GUESA |
|
58.90 |
- |
| 8 |
Munoz, Isabella M (9)
|
GUESA |
|
1:00.85 |
- |
| 9 |
Ropp, Lena W (9)
|
GUBATS |
|
1:08.16 |
- |
| 10 |
Williams, Ava R (9)
|
GUESA |
|
1:18.68 |
- |
|
Bicknell, Josephine F (9)
|
GUESA |
|
DQ |
- |
|
Flake, Rilee D (9)
|
GUNEHA |
44.66 |
DQ |
- |