| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nguyen, Tom T (10)
|
GUESCA |
41.07 |
38.03 |
-3.04 |
| 2 |
Briggs, Jude D (9)
|
GULSST |
38.99 |
39.14 |
0.15 |
| 3 |
Potter, Riley J (10)
|
GUKATY |
46.00 |
39.84 |
-6.16 |
| 4 |
Roberts, Karim M (10)
|
GUPACK |
48.35 |
40.90 |
-7.45 |
| 5 |
Wang, Roy W (10)
|
GUPEAK |
49.46 |
41.31 |
-8.15 |
| 6 |
Nguyen, Harrison K (8)
|
GUESCA |
43.32 |
41.35 |
-1.97 |
| 7 |
Dang, Ethan T (9)
|
GUPEAK |
|
42.63 |
- |
| 8 |
Gu, Allen * (9)
|
GUPEAK |
|
42.82 |
- |
| 9 |
Bezdek, Dylan M (10)
|
GUPEAK |
45.46 |
43.97 |
-1.49 |
| 10 |
Watkins, Michael E (10)
|
GUPEAK |
|
45.16 |
- |
| 11 |
Samuels, Ethan L (10)
|
GUPACK |
|
46.46 |
- |
| 12 |
Keck, Richard J (9)
|
GUPEAK |
47.54 |
46.63 |
-0.91 |
| 13 |
Lanigan, Daniel B (10)
|
GUAMBU |
|
47.82 |
- |
| 14 |
Wildermuth, Benjamin R (10)
|
GUPACK |
|
48.37 |
- |
| 14 |
Davila, Santiago (10)
|
GUPACK |
|
48.37 |
- |
| 16 |
Wright, Matthew S (10)
|
GUPACK |
53.13 |
48.51 |
-4.62 |
| 17 |
Canovas, William W (10)
|
GUPACK |
|
49.05 |
- |
| 18 |
Castellanos, Hunter J (10)
|
GUKATY |
|
49.26 |
- |
| 19 |
Shah, Jonathan K (9)
|
GUPACK |
54.93 |
49.38 |
-5.55 |
| 20 |
Li, Colton * (9)
|
GUPEAK |
|
50.99 |
- |
| 21 |
Saraya, Ibrahim Jr M (10)
|
GUESCA |
|
53.15 |
- |
| 22 |
Roberts, Omar S (10)
|
GUPACK |
58.37 |
53.33 |
-5.04 |
| 23 |
Hutto, Jarrett T (10)
|
GUKATY |
51.33 |
56.45 |
5.12 |
| 24 |
Weaver, Jacob E (10)
|
GUPEAK |
57.66 |
56.63 |
-1.03 |
| 25 |
Wesley, Caleb M (10)
|
GUPEAK |
1:14.07 |
58.53 |
-15.54 |
| 26 |
Doan, Dreyden C (9)
|
GULSST |
53.46 |
1:04.32 |
10.86 |