| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McNames, Owen J (10)
|
GUPACE |
31.17 |
34.79 |
3.62 |
| 2 |
Ortiz, Noah J (10)
|
GUPEAK |
39.61 |
35.36 |
-4.25 |
| 3 |
Camarena, Alejandro * (9)
|
GUPEAK |
36.86 |
35.89 |
-0.97 |
| 4 |
Briggs, Jude D (9)
|
GULSST |
35.93 |
36.27 |
0.34 |
| 5 |
Wiesenborn, Carson S (10)
|
GUKATY |
39.73 |
37.69 |
-2.04 |
| 6 |
Fu, Edward J (10)
|
GUPEAK |
|
38.37 |
- |
| 7 |
Galvez, Vincent R (9)
|
GUESCA |
34.34 |
38.76 |
4.42 |
| 8 |
Silva, Diego A (10)
|
GUESCA |
36.07 |
39.03 |
2.96 |
| 9 |
Danican, Mathis Y (8)
|
GUESCA |
34.17 |
39.38 |
5.21 |
| 10 |
Kim, Peter S (10)
|
GUKATY |
35.85 |
39.79 |
3.94 |
| 11 |
Keck, Richard J (9)
|
GUPEAK |
44.44 |
40.09 |
-4.35 |
| 12 |
Dang, Ethan T (9)
|
GUPEAK |
|
40.97 |
- |
| 13 |
Thurman, Tyler L (10)
|
GUESCA |
36.68 |
41.53 |
4.85 |
| 14 |
Gao, Edwin T (10)
|
GUPEAK |
|
42.39 |
- |
| 15 |
Damiani, Stephen S (10)
|
GUBATS |
36.20 |
42.53 |
6.33 |
| 16 |
Chen, Jason Bowen * (10)
|
GUPEAK |
43.14 |
43.01 |
-0.13 |
| 17 |
Gao, Erick T (10)
|
GUPEAK |
|
43.26 |
- |
| 18 |
Doan, Dreyden C (9)
|
GULSST |
38.71 |
44.18 |
5.47 |
| 19 |
Hutto, Jarrett T (10)
|
GUKATY |
39.15 |
44.22 |
5.07 |