| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kizek, Frederica A (18)
|
GUPFL |
2:27.90 |
2:34.54 |
6.64 |
| 2 |
Guice, Richelle J (17)
|
GUUN-B |
2:36.73 |
2:36.80 |
0.07 |
| 3 |
Xiao, Emma Y (15)
|
GUKATY |
2:35.45 |
2:38.56 |
3.11 |
| 4 |
Barton, Samantha V (15)
|
GUPFL |
2:43.21 |
2:40.99 |
-2.22 |
| 5 |
Lu, Sarah (15)
|
GUKATY |
2:34.89 |
2:41.54 |
6.65 |
| 6 |
Jaco, Reagan L (15)
|
GUSSAN |
2:24.59 |
2:42.27 |
17.68 |
| 7 |
Singh, Isabelle H (15)
|
GUPACK |
2:44.91 |
2:43.41 |
-1.50 |
| 8 |
Goebel, Riley K (15)
|
GUBTA |
2:42.30 |
2:45.35 |
3.05 |
| 9 |
Beardmore, Ashley K (15)
|
GUSSAN |
2:29.37 |
2:47.01 |
17.64 |
| 10 |
Collier, Rachel E (16)
|
GUKATY |
2:31.77 |
2:47.08 |
15.31 |
| 11 |
Grigar, Valerie M (16)
|
GUKATY |
2:54.07 |
2:48.82 |
-5.25 |
| 12 |
Salge, Kaleigh C (16)
|
GUNOCH |
2:51.46 |
2:49.98 |
-1.48 |
| 13 |
Killinger, Kaitlyn (16)
|
GUKATY |
2:46.40 |
2:51.92 |
5.52 |
| 14 |
Bernal Gomez, Paula A (16)
|
GUAGS |
2:48.84 |
2:52.72 |
3.88 |
| 15 |
Wells, Margaret D (15)
|
GUMARC |
|
2:53.70 |
- |
| 16 |
Spencer, Bo L (15)
|
GUKATY |
3:01.88 |
2:54.82 |
-7.06 |
| 17 |
Szafranski, Sarah G (17)
|
GURICE |
3:00.21 |
2:59.89 |
-0.32 |
| 18 |
Musselman, Madison P (15)
|
GUKATY |
2:53.02 |
3:00.57 |
7.55 |
| 19 |
Kalman, Natalie P (15)
|
GUBTA |
2:50.71 |
3:00.85 |
10.14 |
| 20 |
Smith, Hannah C (15)
|
GUMARC |
|
3:01.78 |
- |
| 21 |
Knoerzer, Lexi L (15)
|
GUBTA |
3:05.17 |
3:03.46 |
-1.71 |
| 22 |
Sacco, Claire E (15)
|
GUBTA |
2:43.09 |
3:06.63 |
23.54 |
| 23 |
Olson, Kyleigh E (16)
|
GUTASC |
3:12.06 |
3:09.94 |
-2.12 |
| 24 |
Pigg, Emily M (15)
|
GUMARC |
3:21.48 |
3:09.98 |
-11.50 |
| 25 |
Watts, Emma F (15)
|
GUPFL |
3:06.79 |
3:10.89 |
4.10 |
|
Pierce, Morgan A (16)
|
GUBATS |
2:50.87 |
DQ |
- |