| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nwaeze, Zuilda N (14)
|
MDCAA |
24.86 |
25.01 |
0.15 |
| 2 |
Young, Lauren G (18)
|
PVMACH |
24.44 |
25.06 |
0.62 |
| 3 |
Boggs, Jasmine L (13)
|
PVBWST |
25.80 |
25.30 |
-0.50 |
| 4 |
Haise, Sophie A (16)
|
VAACAC |
24.67 |
25.38 |
0.71 |
| 5 |
Eborn, Janaa A (16)
|
PVDCPR |
25.08 |
25.53 |
0.45 |
| 6 |
Cruz, Agnes E (14)
|
NCEAC |
25.25 |
25.61 |
0.36 |
| 7 |
Gibbons, Delaney F (14)
|
VA757 |
25.24 |
25.64 |
0.40 |
| 8 |
Cowperthwaite, Riley E (16)
|
MDFSC |
25.39 |
25.72 |
0.33 |
| 9 |
Harrison, Elena S (13)
|
PVNCAP |
25.69 |
25.78 |
0.09 |
| 10 |
Fish, Shelby M (13)
|
MDCAA |
25.25 |
25.81 |
0.56 |
| 11 |
Jones, Ava E (15)
|
PVMACH |
26.58 |
25.82 |
-0.76 |
| 12 |
Rothrock, Jessica L (15)
|
MDCAA |
25.70 |
25.91 |
0.21 |
| 13 |
Stefanowicz, Arabella L (15)
|
AMSVSC |
25.79 |
26.05 |
0.26 |
| 14 |
Forman, Faith K (15)
|
MALAC |
26.32 |
26.07 |
-0.25 |
| 15 |
Rider, Mackenzie A (15)
|
NCEAC |
26.21 |
26.17 |
-0.04 |
| 15 |
Lenham, Nora K (14)
|
MDASC |
27.02 |
26.17 |
-0.85 |
| 17 |
Pham, Jordan E (16)
|
PVBWST |
25.70 |
26.22 |
0.52 |
| 18 |
Jacobs, Lillyan K (16)
|
PVMACH |
26.80 |
26.34 |
-0.46 |
| 19 |
Young, Julia K (18)
|
PVMACH |
26.39 |
26.35 |
-0.04 |
| 20 |
Colvard, Chloe T (13)
|
MDFAST |
26.46 |
26.42 |
-0.04 |
| 21 |
Gooding, Emily A (15)
|
PVSDS |
56.84 |
26.52 |
-30.32 |
| 22 |
Krouse, Mackenna R (17)
|
MDFSC |
26.50 |
26.80 |
0.30 |
| 23 |
Keller, Sophia E (15)
|
MDASC |
26.65 |
26.94 |
0.29 |