| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Coe, Angela A (15)
|
ILHEAT |
23.36 |
24.11 |
0.75 |
| 2 |
Gandhi, Arya S (14)
|
ILBNY |
26.53 |
26.19 |
-0.34 |
| 3 |
Coe, Jasmine M (17)
|
ILHEAT |
26.77 |
26.57 |
-0.20 |
| 4 |
Terry, Olivia R (13)
|
ILHEAT |
27.33 |
27.40 |
0.07 |
| 5 |
Mansfield, Madilynn E (13)
|
ILYDSC |
28.10 |
27.99 |
-0.11 |
| 6 |
Mousty, Kyleigh E (13)
|
ILPAWW |
29.46 |
28.28 |
-1.18 |
| 7 |
Mueller, Anna T (13)
|
ILBNY |
28.98 |
28.29 |
-0.69 |
| 8 |
Fuller, Payton J (14)
|
ILHEAT |
27.68 |
28.34 |
0.66 |
| 9 |
Dempsey, Olivia E (13)
|
ILHEAT |
28.79 |
28.91 |
0.12 |
| 10 |
Gilbertz, Lin R (13)
|
ILHEAT |
30.96 |
29.96 |
-1.00 |
| 11 |
Steider, Catherine L (13)
|
ILFFSC |
|
30.29 |
- |
| 12 |
Gulley, Georgette G (13)
|
ILACAD |
30.96 |
32.01 |
1.05 |
| 13 |
Lawton, Elizabeth S (13)
|
ILMARL |
32.15 |
32.46 |
0.31 |
| 14 |
Tibbitts, Jaycee G (14)
|
ILFFSC |
38.82 |
34.74 |
-4.08 |
| 15 |
Harrison, Emma R (13)
|
ILFFSC |
34.12 |
34.83 |
0.71 |
| 16 |
Bishop, Devan M (16)
|
ILFFSC |
37.49 |
35.66 |
-1.83 |
| 17 |
Winters, Kaylin D (13)
|
ILOCS |
37.65 |
36.43 |
-1.22 |