| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pawa, Anika R (11)
|
NCEAC |
35.50 |
35.03 |
-0.47 |
| 2 |
Mizuba, Hannah R (11)
|
VASFY |
35.05 |
36.37 |
1.32 |
| 3 |
Martin, Hannah C (11)
|
VAHOKI |
35.75 |
36.58 |
0.83 |
| 4 |
Murtaugh, Lindsay E (11)
|
VAGATR |
36.59 |
37.72 |
1.13 |
| 5 |
Sylvester-Johnson, Jillian B (11)
|
VAGATR |
37.97 |
38.61 |
0.64 |
| 6 |
Fernando, Scarlett O (11)
|
NCEAC |
38.98 |
39.11 |
0.13 |
| 7 |
Carter, Emmi D (11)
|
VACCA |
38.16 |
39.71 |
1.55 |
| 8 |
Mitias, Ava M (11)
|
WVYKVK |
37.24 |
40.27 |
3.03 |
| 9 |
Byrnes, Jia L (11)
|
VAGATR |
40.45 |
42.32 |
1.87 |
| 10 |
Sanford, Anna M (11)
|
VAHOKI |
40.95 |
43.95 |
3.00 |
| 11 |
Putallaz, Riley I (11)
|
VAHOKI |
46.14 |
44.01 |
-2.13 |
| 12 |
Viar, Olivia B (11)
|
VACCA |
41.53 |
45.21 |
3.68 |
| 13 |
Wohlford, Emma T (11)
|
VAGATR |
47.01 |
47.57 |
0.56 |
| 14 |
Ferreira, Clara (11)
|
VAHOKI |
46.71 |
50.40 |
3.69 |
| 15 |
Furukawa, Minami F (11)
|
VAHOKI |
|
51.39 |
- |
| 16 |
Romeo, Gabrielle V (11)
|
VACCA |
48.04 |
52.81 |
4.77 |
| 17 |
Morris, Tabitha J (11)
|
WVYKVK |
1:00.03 |
59.27 |
-0.76 |