| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Grim, Evelyn M (12)
|
VAHOKI |
30.31 |
30.44 |
0.13 |
| 2 |
Shen, Esther Y (12)
|
VAHOKI |
31.26 |
31.67 |
0.41 |
| 3 |
Agh, Lelle (12)
|
VAGATR |
34.10 |
32.87 |
-1.23 |
| 4 |
Edwards, Natalie M (12)
|
VAHOKI |
33.69 |
32.89 |
-0.80 |
| 5 |
Simkins, Elizabeth J (12)
|
VACCA |
33.42 |
33.52 |
0.10 |
| 5 |
Lewis, Kaitlyn C (12)
|
NCEAC |
32.97 |
33.52 |
0.55 |
| 7 |
Jones, Shelby L (12)
|
VAHOKI |
35.57 |
34.07 |
-1.50 |
| 8 |
Coleman, Tess H (12)
|
VACCA |
34.58 |
34.29 |
-0.29 |
| 9 |
Bonadeo, Lucy E (12)
|
VAHOKI |
41.09 |
41.41 |
0.32 |
|
Viar, Annaliese R (12)
|
VACCA |
35.44 |
NS |
- |
|
Schaefer, Madeline E (12)
|
WVYKVK |
33.59 |
NS |
- |