| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stanley, Shelby A (18)
|
VAGATR |
2:36.04 |
2:49.38 |
13.34 |
| 2 |
Minnes, Asia C (15)
|
VAUN |
2:35.22 |
2:51.12 |
15.90 |
| 3 |
Fleenor, Anna Clare C (17)
|
VASFY |
2:42.39 |
2:53.05 |
10.66 |
| 4 |
Summerlin, Caroline A (15)
|
VAGATR |
2:43.41 |
2:56.09 |
12.68 |
| 5 |
Harris, Suzanne E (15)
|
VAGATR |
2:41.53 |
2:59.12 |
17.59 |
| 6 |
Muzzy, Ava M (13)
|
VAGATR |
2:42.74 |
2:59.16 |
16.42 |
| 7 |
Whitman, Ella M (15)
|
NCEAC |
2:57.12 |
3:04.94 |
7.82 |
| 8 |
Rieker, Julianna E (15)
|
NCEAC |
|
3:08.05 |
- |
| 9 |
Whitlow, Lane O (14)
|
VAGATR |
2:57.57 |
3:08.88 |
11.31 |
| 10 |
Bleecker, Juliana M (16)
|
VACCA |
2:56.28 |
3:11.67 |
15.39 |
| 11 |
Robinson, Tatum A (15)
|
VAHOKI |
2:46.46 |
3:12.66 |
26.20 |
| 12 |
Atkins, Mary G (14)
|
VAGATR |
2:58.20 |
3:13.89 |
15.69 |
| 13 |
Fillo, Grace E (13)
|
VAHOKI |
3:21.52 |
3:17.00 |
-4.52 |
| 14 |
Skinner, Samantha R (14)
|
VAHOKI |
3:26.54 |
3:17.19 |
-9.35 |
| 15 |
Lawrence, Peyton E (14)
|
VASFY |
3:07.82 |
3:19.92 |
12.10 |
| 16 |
St. Jean, Kathryn A (14)
|
WVYKVK |
3:11.30 |
3:24.00 |
12.70 |
| 17 |
Wilkes, Carly A (15)
|
VASFY |
3:19.12 |
3:36.84 |
17.72 |
| 18 |
Blake, Lily B (14)
|
WVYKVK |
3:34.37 |
3:38.80 |
4.43 |
| 19 |
Mann, Kenzie B (14)
|
NCEAC |
3:54.32 |
3:46.85 |
-7.47 |
| 20 |
Hall, Adrianna E (14)
|
VASFY |
|
3:50.11 |
- |
| 21 |
Batista, Anna C (15)
|
VACCA |
3:53.95 |
3:50.14 |
-3.81 |
| 22 |
Ayers, Ansley E (13)
|
VACCA |
3:40.83 |
3:50.17 |
9.34 |
| 23 |
Hodge, Emma C (13)
|
VACCA |
3:40.79 |
3:50.46 |
9.67 |
| 24 |
Sudweeks, Erin (17)
|
VAHOKI |
3:35.77 |
3:50.88 |
15.11 |