| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mizuba, Hannah R (12)
|
VASFY |
2:36.81 |
2:27.09 |
-9.72 |
| 2 |
Santucci, Elle M (12)
|
PVBWST |
2:41.73 |
2:27.52 |
-14.21 |
| 3 |
Carr, Samantha M (12)
|
PVBWST |
2:28.35 |
2:27.81 |
-0.54 |
| 4 |
Gorby, Tessa L (12)
|
VAGATR |
2:42.28 |
2:30.16 |
-12.12 |
| 5 |
Alston, Elizabeth D (12)
|
PVMACH |
2:46.30 |
2:33.99 |
-12.31 |
| 6 |
Swanson, Esther M (12)
|
PVMACH |
2:40.85 |
2:34.67 |
-6.18 |
| 7 |
Du, Madelyn (12)
|
PVMACH |
2:36.23 |
2:37.46 |
1.23 |
| 8 |
Janczyk, Megan D (12)
|
PVMACH |
2:38.90 |
2:40.97 |
2.07 |
| 9 |
Martin, Hannah C (12)
|
VAHOKI |
2:41.62 |
2:41.18 |
-0.44 |
| 10 |
Bishop, Evanna K (12)
|
PVMACH |
2:45.41 |
2:42.08 |
-3.33 |
| 11 |
Hodge, Anna M (12)
|
VACCA |
2:42.64 |
2:43.64 |
1.00 |
| 12 |
Viar, Annaliese R (12)
|
VACCA |
2:49.32 |
2:43.79 |
-5.53 |
| 13 |
Schumacher, Karly R (12)
|
PVMACH |
2:51.41 |
2:46.30 |
-5.11 |
| 14 |
Fassacesia, Giada L (12)
|
PVBWST |
2:51.32 |
2:48.26 |
-3.06 |
| 15 |
Couric, Rebecca B (12)
|
VAGATR |
3:02.56 |
2:48.72 |
-13.84 |
| 16 |
Conforti-Johnson, Sophia K (12)
|
VACCA |
2:52.85 |
2:50.55 |
-2.30 |
| 17 |
Hanlin, Carly A (12)
|
PVBWST |
3:03.92 |
2:54.25 |
-9.67 |
| 18 |
Nicholson, Natalie N (12)
|
PVBWST |
3:12.16 |
2:55.44 |
-16.72 |
| 19 |
Skorupski, Reagan M (12)
|
PVBWST |
3:20.49 |
2:56.50 |
-23.99 |
| 20 |
Ladd, Kennedy M (12)
|
PVMACH |
3:59.40 |
2:57.74 |
-61.66 |
| 21 |
Kadela, Ann E (12)
|
VACCA |
|
3:02.78 |
- |
|
Williams, Maeve A (12)
|
PVMACH |
2:47.37 |
NS |
- |