| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nawrocki, Sophie L (14)
|
NEPVAA |
26.52 |
26.18 |
-0.34 |
| 2 |
Pouliot, Claire A (13)
|
NEPVAA |
26.45 |
27.24 |
0.79 |
| 3 |
Melnik, Margaret W (14)
|
NEONE |
26.70 |
27.45 |
0.75 |
| 4 |
Chebotarev, Lara (14)
|
NEPVAA |
26.93 |
27.70 |
0.77 |
| 5 |
Erofeyev, Evelyn (14)
|
NEPVAA |
27.59 |
27.78 |
0.19 |
| 6 |
Zuccalo, Mia K (13)
|
NEUN |
27.66 |
28.34 |
0.68 |
| 7 |
Riley, Amanda M (13)
|
NEPVAA |
28.46 |
28.50 |
0.04 |
| 8 |
Bencivenga, Mia A (14)
|
NEUN |
29.09 |
29.01 |
-0.08 |
| 9 |
Rumlow, Hailey J (13)
|
NEUN |
28.52 |
29.08 |
0.56 |
| 10 |
Goncalves-Korny, Abigayle V (13)
|
NEPVAA |
28.49 |
29.27 |
0.78 |
| 11 |
Hopkins, Sabrina M (13)
|
NEHRY |
28.71 |
29.68 |
0.97 |
| 12 |
Nubile, Lauren C (14)
|
NEPVAA |
29.87 |
29.80 |
-0.07 |
| 13 |
Schettini, Alissa R (13)
|
NEUN |
29.50 |
30.00 |
0.50 |
| 14 |
Weppler, Claire D (14)
|
NEPVAA |
29.84 |
30.72 |
0.88 |
| 15 |
Rueli, Gabrielle C (14)
|
NEPVAA |
31.13 |
30.75 |
-0.38 |
| 16 |
Medaugh, Ava M (13)
|
NEHRY |
30.41 |
30.80 |
0.39 |
| 17 |
Adams-Sternal, Frankie A (13)
|
NEHRY |
30.05 |
30.84 |
0.79 |
| 18 |
Stebbins, Averi O (13)
|
NEPVAA |
31.38 |
31.44 |
0.06 |
| 19 |
Richards, Avery M (14)
|
NEPVAA |
|
31.57 |
- |
| 20 |
Pacheco, Felicia L (13)
|
NEPVAA |
33.80 |
34.42 |
0.62 |
| 21 |
LaTour, Iveleanice (13)
|
NEHYV |
37.31 |
34.90 |
-2.41 |
| 22 |
Wyman, Julia M (13)
|
NEHRY |
37.68 |
37.91 |
0.23 |