| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Xu, Jingxing (8)
|
NIJETS |
25.20 |
24.03 |
-1.17 |
| 2 |
Checksfield, Charlie M (10)
|
NIJETS |
25.97 |
24.71 |
-1.26 |
| 3 |
Dorsey, Caitlin A (10)
|
NIJETS |
28.68 |
25.47 |
-3.21 |
| 4 |
Cavallerano, Sawyer Q (8)
|
NIJETS |
26.57 |
25.89 |
-0.68 |
| 5 |
Mekker, Ella B (8)
|
NIJETS |
|
26.38 |
- |
| 6 |
Andrianos, Alivia K (10)
|
NIJETS |
36.22 |
28.20 |
-8.02 |
| 7 |
DeStephano, Isabella G (10)
|
NIJETS |
34.09 |
28.61 |
-5.48 |
| 8 |
Atherton, Alexander S (8)
|
NIJETS |
31.33 |
29.19 |
-2.14 |
| 9 |
Woodworth, Meghan M (10)
|
NIJETS |
29.56 |
31.60 |
2.04 |
| 10 |
Akley, Benjamin J (8)
|
NIJETS |
36.57 |
31.93 |
-4.64 |
| 11 |
Atherton, Adelaide G (8)
|
NIJETS |
34.50 |
32.47 |
-2.03 |
| 12 |
Musci, Ava M (9)
|
NIJETS |
40.71 |
35.08 |
-5.63 |
| 13 |
Lawrence, Ava K (8)
|
NIJETS |
38.16 |
37.99 |
-0.17 |
|
Latham, Morgan P (8)
|
NIJETS |
|
DQ |
- |
|
Shutts, Bryce A (8)
|
NIJETS |
33.66 |
DQ |
- |
|
Vavra, Addison E (10)
|
NIJETS |
26.10 |
NS |
- |