| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
DeMario, Emma R (15)
|
MDCCY |
|
31.48 |
- |
| 2 |
Dworkin, Grace (15)
|
MDMSC |
41.96 |
31.88 |
-10.08 |
| 3 |
Holsey, Lily * (16)
|
MDCATY |
33.04 |
32.24 |
-0.80 |
| 4 |
Woo, Candace L (16)
|
MDCAA |
33.07 |
32.50 |
-0.57 |
| 5 |
Nagle, Ashleigh M (17)
|
MDCAA |
33.49 |
32.53 |
-0.96 |
| 6 |
St. Pierre, Shannon M (15)
|
MDCCY |
|
32.82 |
- |
| 7 |
Melick, Abigail G (15)
|
MDBAAC |
32.37 |
33.20 |
0.83 |
| 8 |
Nelson, Sarah L (16)
|
MDBAAC |
33.25 |
33.90 |
0.65 |
| 9 |
Dongarra, Isabella A (16)
|
MDCATY |
35.41 |
34.39 |
-1.02 |
| 10 |
Bryant, Caroline C (15)
|
MDCATY |
|
34.68 |
- |
| 11 |
Amsler, Kendall F (15)
|
MDBAAC |
32.54 |
35.40 |
2.86 |
| 12 |
Marchionni, Luce (15)
|
MDMSC |
36.46 |
37.55 |
1.09 |
| 13 |
Schlichting, Kelsie A (15)
|
MDCAA |
36.33 |
37.68 |
1.35 |
|
Gapaz, Patrice (16)
|
MDCGA |
32.71 |
NS |
- |
|
Dibert, Julianna C (16)
|
MDHOCO |
33.25 |
NS |
- |