| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stamos, Guy C (10)
|
GULSST |
34.12 |
33.83 |
-0.29 |
| 2 |
Toole, Owen S (10)
|
GUESA |
36.13 |
34.56 |
-1.57 |
| 3 |
Faerber, Caden D (9)
|
GUESA |
37.13 |
34.86 |
-2.27 |
| 4 |
Foltz, Casey J (10)
|
GUESA |
36.71 |
35.71 |
-1.00 |
| 5 |
Miguez, Ethan J (9)
|
GUESA |
39.27 |
35.81 |
-3.46 |
| 6 |
Nguyen, Maddox L (10)
|
GULSST |
39.47 |
40.97 |
1.50 |
| 7 |
Bender, Isaiah C (9)
|
GUSWIM |
43.35 |
43.20 |
-0.15 |
| 8 |
Abad, Ryland D (10)
|
GUSWIM |
47.39 |
45.64 |
-1.75 |
| 9 |
Samonte, Connor T (10)
|
GULSST |
52.11 |
45.70 |
-6.41 |
| 10 |
Hearne, Jonah C (9)
|
GUSWIM |
48.52 |
47.43 |
-1.09 |
| 11 |
Nguyen, Ashton N (10)
|
GULSST |
49.26 |
47.50 |
-1.76 |
| 12 |
Elnasr, Adam Z (10)
|
GUSWIM |
48.48 |
48.67 |
0.19 |
| 13 |
Duong, Nathan (10)
|
GULSST |
49.65 |
49.68 |
0.03 |
| 14 |
Williams, Joseph D (9)
|
GUESA |
56.20 |
53.02 |
-3.18 |
| 15 |
Downs, Jonathan C (9)
|
GUSPA |
57.72 |
53.46 |
-4.26 |
| 16 |
Frausto, Dominick J (10)
|
GUESA |
53.93 |
53.64 |
-0.29 |
| 17 |
Andrade, Christian N (10)
|
GUSWIM |
52.06 |
59.36 |
7.30 |
| 18 |
Borja, Nicolas A (10)
|
GUESA |
1:01.47 |
1:01.57 |
0.10 |
| 19 |
Andrade, Jean Paul N (10)
|
GUSWIM |
52.65 |
1:02.17 |
9.52 |
| 20 |
Nguyen, Ayden N (10)
|
GULSST |
56.92 |
1:03.33 |
6.41 |
|
Samonte, Cameron T (9)
|
GULSST |
1:17.65 |
DQ |
- |
|
Fu, Tanghao D (10)
|
GUSPA |
|
DQ |
- |
|
Thomas, Mason C (10)
|
GUSPA |
|
DQ |
- |