| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
White, Emily G (12)
|
MANRG |
35.40 |
32.09 |
-3.31 |
| 2 |
Luetke, Jordan (12)
|
MAUN B |
|
32.73 |
- |
| 3 |
Kouser, Kaitlyn M (11)
|
MAUN B |
|
33.53 |
- |
| 4 |
Donahoe, Cheyenne M (11)
|
MANRG |
33.50 |
33.56 |
0.06 |
| 5 |
Schaenen, Nola A (11)
|
MAFSA |
36.32 |
34.35 |
-1.97 |
| 6 |
Ammon, Isabella R (11)
|
MAFSA |
35.92 |
35.27 |
-0.65 |
| 7 |
Jaskiewicz, Kaitlyn M (11)
|
MANRG |
40.56 |
37.28 |
-3.28 |
| 8 |
Sherman, Gabriella L (12)
|
MAFSA |
35.91 |
37.62 |
1.71 |
| 9 |
Montvydas, Sophia R (11)
|
MANRG |
42.32 |
40.01 |
-2.31 |
| 10 |
Pappas, LucaRose N (11)
|
MAFSA |
53.84 |
49.34 |
-4.50 |
|
Lin, Ava A (11)
|
MAFSA |
42.91 |
NS |
- |